In Vitro Anti-Acetylcholinesterase Activity of Dichloromethane Leaf Extracts of Carphalea glaucescens in Chilo partellus Larvae

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Date
2016
Authors
Njoroge, A.W.
Ngugi, M.P.
Aliyu, U.
Matheri, F.
Gitahi, M.S.
Mwangi, M.B.
Njagi, M.J.
Mworia, J.K.
Ngure, G.M.
Journal Title
Journal ISSN
Volume Title
Publisher
OMICS International
Abstract
Acetlycholinesterase (AChE) hydrolyses the neurotransmitter acetylcholine resulting in the termination of nerve impulse at the synapse. Anti-acetylcholinesterase activities stop the passage of the nerve impulse at the synapse resulting in continuous stimulation which can lead to death. The manufacturers of many pesticides target the AChE because it interferes with the passage of the nerve impulse. In vitro study revealed that DCM leaf extract of Carphalea glaucescens has anti-acetylcholinesterase activity against crude acetlycholinesterase (AChE) enzyme extracted from Chilo partellus and an IC50 of 12.02 mg/ml was calculated. After qualitative phytochemical screening was carried out the phytochemicals which were present were tannins, phenols, flavonoids, steroids, terpenoids and alkaloids
Description
Research Article
Keywords
Chilo partellu, Carphalea glaucescens, Anti-acetylcholinesterase
Citation
Anne NW, Piero NM, Umar A, Felix M, Maina GS, et al. (2016) In Vitro Anti-Acetylcholinesterase Activity of Dichloromethane Leaf Extracts of Carphalea glaucescens in Chilo partellus Larvae. Biochem Anal Biochem 5: 264. doi:10.4172/2161-1009.1000264