Analgesic Potential of Dichloromethane Leaf Extracts of Eucalyptus Globulus (Labill) and Senna Didymobotrya (Fresenius) in Mice Models

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dc.contributor.authorMworia, Joseph Kiambi
dc.contributor.authorKibiti, Cromwell Mwiti
dc.contributor.authorNgeranwa, Joseph JN
dc.contributor.authorNgugi, Mathew Piero
dc.date.accessioned2020-07-14T14:37:32Z
dc.date.available2020-07-14T14:37:32Z
dc.date.issued2020
dc.descriptionA research article published in Journal of Herbmed Pharmacology. Fulltext: http://www.herbmedpharmacol.com/Files/Inpress/jhp-36637.pdfen_US
dc.description.abstractIntroduction: Pain is managed using conventional drugs like paracetamol, aspirin and diclofenac among others. Synthetic drugs have many side effects. This study aimed at evaluating the analgesic potential of the dichloromethane leaf extracts of Eucalyptus globulus and Senna didymobotrya in mice. Methods: The dichloromethane leaf extracts of E. globulus and S. didymobotrya were subjected to quantitative phytochemical analysis using gas chromatography-mass spectrophotometry (GC-MS). In vivo analgesic evaluation comprised of nine groups of animals (Swiss albino mice): normal, positive, negative control and six experimental groups that received 25, 50, 100, 150, 200 and 250 mg/kg body weight of each plant extract intraperitoneally. Thirty minutes later, they were injected with 0.01 mL of 2.5% formalin. The animals in positive control group were administered diclofenac (15 mg/kg) and formalin, the normal control mice received 3% dimethyl sulfoxide (DMSO) in normal saline, while the negative group received DMSO in normal saline and formalin. All the doses were administered intraperitoneally. The duration of shaking and licking of the injected paw was scored and analyzed. Results: The analysis revealed that E. globulus contained alpha-pinenes, endo-fenchol, α-eudesmol, myrcene, camphene, alpha-phellandrene, limonene, and camphor while S. dymobotrya possessed camphene, alpha-phellandrene, limonene, and camphor. In the late phase, E. globulus at the doses of 25, 50, 100, 150, 200 and 250 mg/kg reduced the paw licking time by 34.03%, 60.79%, 84.33 %, 90.65%, 94.49%, 98.52%, respectively while S. didymobotrya extract reduced the paw licking time by 26.48%, 32.96%, 87.04%, 91.27%, 93.40%, 90.97%, and 96.82%, respectively. Conclusion: The results of this study validate and support the traditional uses of these plants as analgesics.en_US
dc.identifier.citationJoseph KM, Cromwell MK, Joseph NN, Mathew PN. Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models. J Herbmed Pharmacol. 2020;9(4):391-399. doi: 10.34172/jhp.2020.49.en_US
dc.identifier.issn2345-5004
dc.identifier.urihttp://www.herbmedpharmacol.com/Files/Inpress/jhp-36637.pdf
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/20222
dc.language.isoenen_US
dc.publisherJournal of Herbmed Pharmacologyen_US
dc.subjectAnalgesicen_US
dc.subjectdichloromethane leaf extractsen_US
dc.subjectSenna didymobotryaen_US
dc.subjectmice modelsen_US
dc.titleAnalgesic Potential of Dichloromethane Leaf Extracts of Eucalyptus Globulus (Labill) and Senna Didymobotrya (Fresenius) in Mice Modelsen_US
dc.typeArticleen_US
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