Analgesic Potential of Dichloromethane Leaf Extracts of Eucalyptus Globulus (Labill) and Senna Didymobotrya (Fresenius) in Mice Models
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Date
2020
Authors
Mworia, Joseph Kiambi
Kibiti, Cromwell Mwiti
Ngeranwa, Joseph JN
Ngugi, Mathew Piero
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Herbmed Pharmacology
Abstract
Introduction: Pain is managed using conventional drugs like paracetamol, aspirin and
diclofenac among others. Synthetic drugs have many side effects. This study aimed at evaluating
the analgesic potential of the dichloromethane leaf extracts of Eucalyptus globulus and Senna
didymobotrya in mice.
Methods: The dichloromethane leaf extracts of E. globulus and S. didymobotrya were subjected
to quantitative phytochemical analysis using gas chromatography-mass spectrophotometry
(GC-MS). In vivo analgesic evaluation comprised of nine groups of animals (Swiss albino
mice): normal, positive, negative control and six experimental groups that received 25, 50, 100,
150, 200 and 250 mg/kg body weight of each plant extract intraperitoneally. Thirty minutes
later, they were injected with 0.01 mL of 2.5% formalin. The animals in positive control group
were administered diclofenac (15 mg/kg) and formalin, the normal control mice received 3%
dimethyl sulfoxide (DMSO) in normal saline, while the negative group received DMSO in
normal saline and formalin. All the doses were administered intraperitoneally. The duration of
shaking and licking of the injected paw was scored and analyzed.
Results: The analysis revealed that E. globulus contained alpha-pinenes, endo-fenchol,
α-eudesmol, myrcene, camphene, alpha-phellandrene, limonene, and camphor while S.
dymobotrya possessed camphene, alpha-phellandrene, limonene, and camphor. In the late
phase, E. globulus at the doses of 25, 50, 100, 150, 200 and 250 mg/kg reduced the paw licking
time by 34.03%, 60.79%, 84.33 %, 90.65%, 94.49%, 98.52%, respectively while S. didymobotrya
extract reduced the paw licking time by 26.48%, 32.96%, 87.04%, 91.27%, 93.40%, 90.97%, and
96.82%, respectively.
Conclusion: The results of this study validate and support the traditional uses of these plants
as analgesics.
Description
A research article published in Journal of Herbmed Pharmacology. Fulltext: http://www.herbmedpharmacol.com/Files/Inpress/jhp-36637.pdf
Keywords
Analgesic, dichloromethane leaf extracts, Senna didymobotrya, mice models
Citation
Joseph KM, Cromwell MK, Joseph NN, Mathew PN. Analgesic potential of dichloromethane leaf extracts of Eucalyptus globulus (Labill) and Senna didymobotrya (Fresenius) in mice models. J Herbmed Pharmacol. 2020;9(4):391-399. doi: 10.34172/jhp.2020.49.