Application and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenya

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dc.contributor.authorDouksouna, Youmma
dc.contributor.authorKwallah, Allan Ole
dc.contributor.authorNyerere, Andrew
dc.contributor.authorRuno, Steven
dc.contributor.authorAmbang, Zachée
dc.date.accessioned2020-10-22T05:36:04Z
dc.date.available2020-10-22T05:36:04Z
dc.date.issued2020
dc.descriptionAn article published in Journal of Plant Pathology & Microbiologyen_US
dc.description.abstractAflatoxigenic fungi are most common filamentous fungi that synthesis aflatoxins and represent the major fungal pathogens to agricultural products. Aflatoxins remain a major threat to global food security, these molecules could be resisted into food during processing and in additional may remain within the food chain. Aflatoxins are carcinogenic, hepatotoxic, mutagenic, teratogenic, can inhibit numerous metabolic systems and immunosuppressive properties. Studies of aflatoxigenic strains can help to enhance strategies control and prevent aflatoxigenic fungi contamination and aflatoxins production in foodstuffs. In this study, isolation of Aspergillus species was based on morphological characteristics including the mycelium growth pattern, color, and properties of fruiting bodies of the fungi. The innovated technique loop-mediated isothermal amplification assay was applied to amplify Norsolorinic Acid gene. The loop-mediated isothermal amplification have been optimized by combination of the rapidity, simplicity and specificity to detect the target genomic DNA in the reactions. The amplification curves monitored by Loopamp realtime Turbidi meter were analyzed in order to distinguish aflatoxigenic and non-aflatoxigenic strains. Overall, the results showed that the loop-mediated isothermal amplification method was effective in detecting aflatoxigenic strains with high specificity of 71.5 % and sensitivity under lower concentrations of DNA. In additional, it was faster than the conventional polymerase chain reaction. The loop-mediated isothermal amplification assay described in this study might be a promising tool for prediction potential threats by aflatoxigenic fungi and aflatoxins risk in food and commoditiesen_US
dc.identifier.citationDouksouna Y, Kwallah AO, Nyerere A, Runo S, Ambang Z (2020) Application and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenya. Plant Pathol Microbiol. 11:491. doi: 10.35248/2157-7471.20.11.491en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/20616
dc.language.isoenen_US
dc.publisherLongdom Publishingen_US
dc.subjectRice grainsen_US
dc.subjectLAMPen_US
dc.subjectRapid detectionen_US
dc.subjectNor-1en_US
dc.subjectAflatoxigenicen_US
dc.titleApplication and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenyaen_US
dc.typeArticleen_US
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