Application and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenya
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Date
2020
Authors
Douksouna, Youmma
Kwallah, Allan Ole
Nyerere, Andrew
Runo, Steven
Ambang, Zachée
Journal Title
Journal ISSN
Volume Title
Publisher
Longdom Publishing
Abstract
Aflatoxigenic fungi are most common filamentous fungi that synthesis aflatoxins and represent the major fungal
pathogens to agricultural products. Aflatoxins remain a major threat to global food security, these molecules could be
resisted into food during processing and in additional may remain within the food chain. Aflatoxins are carcinogenic,
hepatotoxic, mutagenic, teratogenic, can inhibit numerous metabolic systems and immunosuppressive properties.
Studies of aflatoxigenic strains can help to enhance strategies control and prevent aflatoxigenic fungi contamination
and aflatoxins production in foodstuffs. In this study, isolation of Aspergillus species was based on morphological
characteristics including the mycelium growth pattern, color, and properties of fruiting bodies of the fungi. The
innovated technique loop-mediated isothermal amplification assay was applied to amplify Norsolorinic Acid gene.
The loop-mediated isothermal amplification have been optimized by combination of the rapidity, simplicity and
specificity to detect the target genomic DNA in the reactions. The amplification curves monitored by Loopamp realtime Turbidi meter were analyzed in order to distinguish aflatoxigenic and non-aflatoxigenic strains.
Overall, the results showed that the loop-mediated isothermal amplification method was effective in detecting
aflatoxigenic strains with high specificity of 71.5 % and sensitivity under lower concentrations of DNA. In additional,
it was faster than the conventional polymerase chain reaction. The loop-mediated isothermal amplification assay
described in this study might be a promising tool for prediction potential threats by aflatoxigenic fungi and aflatoxins
risk in food and commodities
Description
An article published in Journal of Plant Pathology & Microbiology
Keywords
Rice grains, LAMP, Rapid detection, Nor-1, Aflatoxigenic
Citation
Douksouna Y, Kwallah AO, Nyerere A, Runo S, Ambang Z (2020) Application and Evaluation of the Loop-Mediated Isothermal Amplification assay for the Detection of Aflatoxigenic Fungi Contaminants of Rice Grains in Kenya. Plant Pathol Microbiol. 11:491. doi: 10.35248/2157-7471.20.11.491