Prevalence and Genetic Diversity of HAV and HBV Viruses among Jaundice Patients at Coast General Hospital, Mombasa County, Kenya
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Date
2021
Authors
Kasera, Gordon Ochieng’
Maingi, John M
Onyango, Omondi Kevin
Nyamache, Anthony Kebira
Journal Title
Journal ISSN
Volume Title
Publisher
School of Medical Sciences, Universiti Sains Malaysia
Abstract
Background: Hepatitis A and B causes morbidity and mortality among patients. This
study determined the proportion of hepatitis A, B viruses (HAV, HBV) and genetic diversity of HBV
among jaundice patients at the Coast General Hospital, Mombasa County, Kenya.
Methods: A cross-sectional study was conducted among 222 patients; recruited and
screened for hepatitis B surface antigen (HBsAg) and anti-HAV IgM. Viral deoxyribonucleic acid
(DNA) was extracted from positive samples; partial hepatitis B virus-pol (HBV-pol) gene amplified,
directly sequenced and generated sequences phylogenetically analysed using MEGA X software.
Demographic characteristics were compared in relation to HBV infection using Chi-square.
Results: Forty-seven (21.2%) out of the 222 patients tested positive for HBV while no
HAV was detected. Among those infected, n = 8 (3.6%) were females and n = 39 (17.6%) males.
Forty-five samples amplified and sequenced successfully. However, two samples failed to amplify.
Phylogenetic analysis revealed HBV A1 genotype [n = 35 (74.5%)] was most predominant. A3, B and
C2 genotypes each occurred [n = 1 (0.02%)]. This study revealed co-existence of HBV A3, B and C2
genotypes that have not yet been detected in this region.
Conclusion: HBV A1 genotype remains the predominant genotypes in this region. The
detected HBV prevalence indicates possible high transmission with possibility of increasing trends
of HBV genotypes based on revelation of existence of new genotypes in this region.
Description
Article
Keywords
prevalence, genetic diversity, HAV, HBV, jaundice
Citation
Ochieng’Kasera, G., Maingi, J. M., Onyango, O. K., & Nyamache, A. K. (2021). Prevalence and Genetic Diversity of HAV and HBV Viruses among Jaundice Patients at Coast General Hospital, Mombasa County, Kenya. The Malaysian Journal of Medical Sciences: MJMS, 28(3), 56.