In Vivo Anti-Inflammatory, Anti-Nociceptive, and in Vitro Antioxidant Efficacy, and Acute Oral Toxicity Effects of the Aqueous and Methanolic Stem Bark Extracts of Lonchocarpus Eriocalyx (Harms.)

Simple item page
dc.contributor.authorMoriasi, Gervason Apiri
dc.contributor.authorIreri, Anthony Muriithi
dc.contributor.authorNelson, Elias Mandela
dc.contributor.authorNgugi, Mathew Piero
dc.date.accessioned2023-05-22T08:54:13Z
dc.date.available2023-05-22T08:54:13Z
dc.date.issued2021
dc.descriptionArticleen_US
dc.description.abstractOxidative stress causes and drives many agonising inflammatory conditions, which cause disability, financial burden, and emotional stress. The current anti-inflammatory, analgesic, and antioxidant agents are associated with adverse effects, inaccessibility, high costs, and low efficacies, thereby warranting the need for alternatives, especially from natural sources. Lonchocarpus eriocalyx plant is traditionally used in Kenyan communities to treat various inflammatory and oxidative stress-associated diseases; however, its pharmacologic efficacy and safety have not been empirically validated, hence this study. The in vivo antiinflamatory and antinociceptive efficacy of the aqueous and methanolic stem bark extracts of L. eriocalyx were determined using the xylene-induced ear oedema, and the acetic acid-induced writhing techniques, respectively, in experimental mice. Also, in vitro antioxidant activities of the studied plant extracts were investigated using the Thiobarbituric acid test for lipid peroxidation, 1, 1-diphenyl -2-picrylhydrazyl (DPPH), and Ferric reducing antioxidant power standard assay methods. Moreover, the studied extracts' acute oral toxicity effects were investigated according to the Organisation for Economic Corporation and Development (OECD) guidelines. The studied plant extracts showed significant dose-dependent inhibitions of oedema and writhing, depicting their anti-inflammatory and antinociceptive efficacy. Besides, the extracts revealed significant inhibitions of in vitro lipid peroxidation in varying degrees. Notably, the extracts demonstrated very strong DPPH radical scavenging and ferric-reducing antioxidant efficacies. Furthermore, the two studied plant extracts did not elicit acute oral toxicity, with LD50 values of >2000 mg/kg BW, hence were considered safe. The anti-inflammatory, antinociceptive, and in vitro antioxidant efficacies of these extracts were attributed to antioxidant phytocompounds with diverse pharmacologic effects, especially through the amelioration of oxidative stress. Further studies on the anti-inflammatory, antinociceptive and antioxidant mechanism(s) and isolation and characterisation of responsible compounds are encouraged to spur the development of affordable, accessible, safe, and efficacious drugs.en_US
dc.description.sponsorshipThe technical staff of the Department of Biochemistry, Microbiology, and Biotechnology of Kenyatta University and the Directorate of Research and Innovation of Mount Kenya University.en_US
dc.identifier.citationMoriasi, G. A., Ireri, A. M., Nelson, E. M., & Ngugi, M. P. (2021). In vivo anti-inflammatory, anti-nociceptive, and in vitro antioxidant efficacy, and acute oral toxicity effects of the aqueous and methanolic stem bark extracts of Lonchocarpus eriocalyx (Harms.). Heliyon, 7(5), e07145.en_US
dc.identifier.otherhttps://doi.org/10.1016/j.heliyon.2021.e0
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/25373
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.subjectAnti-inflammatory activityen_US
dc.subjectAntinociceptive activityen_US
dc.subjectAcute oral toxicity effectsen_US
dc.subjectOxidative stressen_US
dc.subjectFerric reducing antioxidant poweren_US
dc.subjectDPPH radical Scavenging activityen_US
dc.subjectLipid peroxidationen_US
dc.subjectLonchocarpus eriocalyxen_US
dc.titleIn Vivo Anti-Inflammatory, Anti-Nociceptive, and in Vitro Antioxidant Efficacy, and Acute Oral Toxicity Effects of the Aqueous and Methanolic Stem Bark Extracts of Lonchocarpus Eriocalyx (Harms.)en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
In Vivo Anti-Inflammatory, Anti-Nociceptive, and in Vitro Antioxidant Efficacy, and Acute Oral Toxicity Effects of the Aqueous and Methanolic Stem Bark Extracts of Lonchocarpus Eriocalyx (Harms.).pdf
Size:
1.03 MB
Format:
Adobe Portable Document Format
Description:
Full text Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Biochemistry and Biotechnology