Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.

dc.contributor.author	Njagi, E.N.M.
dc.contributor.author	Kanyara, J. N.
dc.date.accessioned	2014-01-16T12:24:25Z
dc.date.available	2014-01-16T12:24:25Z
dc.date.issued	2005-04
dc.identifier.citation	Phytother Res. 2005 Apr;19(4):287-90.	en_US
dc.identifier.uri	http://ir-library.ku.ac.ke/handle/123456789/8615
dc.description	DOI: 10.1002/ptr.1536
dc.description.abstract	An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented.	en_US
dc.language.iso	en	en_US
dc.publisher	Phytotherapy Research	en_US
dc.subject	HIV-1
dc.subject	reverse transcriptase assay
dc.subject	medicinal plants
dc.subject	extracts
dc.subject	anti-HIV activity
dc.subject	IC50
dc.title	Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.	en_US
dc.type	Article	en_US