Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.
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Date
2005-04
Authors
Njagi, E.N.M.
Kanyara, J. N.
Journal Title
Journal ISSN
Volume Title
Publisher
Phytotherapy Research
Abstract
An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented.
Description
DOI: 10.1002/ptr.1536
Keywords
HIV-1, reverse transcriptase assay, medicinal plants, extracts, anti-HIV activity, IC50
Citation
Phytother Res. 2005 Apr;19(4):287-90.