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dc.contributor.authorNjagi, E.N.M.
dc.contributor.authorKanyara, J. N.
dc.date.accessioned2014-01-16T12:24:25Z
dc.date.available2014-01-16T12:24:25Z
dc.date.issued2005-04
dc.identifier.citationPhytother Res. 2005 Apr;19(4):287-90.en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/8615
dc.descriptionDOI: 10.1002/ptr.1536
dc.description.abstractAn in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented.en_US
dc.language.isoenen_US
dc.publisherPhytotherapy Researchen_US
dc.subjectHIV-1
dc.subjectreverse transcriptase assay
dc.subjectmedicinal plants
dc.subjectextracts
dc.subjectanti-HIV activity
dc.subjectIC50
dc.titleAnti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.en_US
dc.typeArticleen_US


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