Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay.
| dc.contributor.author | Njagi, E.N.M. | |
| dc.contributor.author | Kanyara, J. N. | |
| dc.date.accessioned | 2014-01-16T12:24:25Z | |
| dc.date.available | 2014-01-16T12:24:25Z | |
| dc.date.issued | 2005-04 | |
| dc.description | DOI: 10.1002/ptr.1536 | |
| dc.description.abstract | An in vitro HIV-1 reverse transcriptase (RT) assay was used for screening of anti-HIV activity of extracts obtained from some Kenyan medicinal plants. The assay utilises [3H]-methyl thymidine triphosphate (dTTP) as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)(12-18)] as the template-primer dimmer. This assay was optimised and standardised with respect to the various experimental parameters in a microtiter plate methodology. The assay was then applied to test for potential antiviral activities of several Kenyan medicinal plant extracts and the concentrations producing 50% inhibition (IC50) of the HIV-1 RT were determined. This assay is described in this report and results obtained with some of the extracts are presented. | en_US |
| dc.identifier.citation | Phytother Res. 2005 Apr;19(4):287-90. | en_US |
| dc.identifier.uri | http://ir-library.ku.ac.ke/handle/123456789/8615 | |
| dc.language.iso | en | en_US |
| dc.publisher | Phytotherapy Research | en_US |
| dc.subject | HIV-1 | |
| dc.subject | reverse transcriptase assay | |
| dc.subject | medicinal plants | |
| dc.subject | extracts | |
| dc.subject | anti-HIV activity | |
| dc.subject | IC50 | |
| dc.title | Anti-HIV-1 activities in extracts from some medicinal plants as assessed in an in vitro biochemical HIV-1 reverse transcriptase assay. | en_US |
| dc.type | Article | en_US |