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dc.contributor.authorTinega, A. N.
dc.contributor.authorPellé, R.
dc.contributor.authorKang'a, S.
dc.contributor.authorGicheru, M. M.
dc.contributor.authorTaracha, E.
dc.contributor.authorNene, V.
dc.contributor.authorGraham, S. P.
dc.date.accessioned2014-01-14T15:08:22Z
dc.date.available2014-01-14T15:08:22Z
dc.date.issued2009
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/8547
dc.descriptiondoi: 10.1016/j.vetimm.2009.01.008.en_US
dc.description.abstractImmunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-gamma ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo.en_US
dc.language.isoenen_US
dc.publisherPubMeden_US
dc.titleFusion of a Cell Penetrating Peptide from HIV-1 TAT to the Theileria Parva Antigen Tp2 Enhances the Stimulation of Bovine CD8 T Cell Responses.en_US
dc.typeArticleen_US


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