Fusion of a Cell Penetrating Peptide from HIV-1 TAT to the Theileria Parva Antigen Tp2 Enhances the Stimulation of Bovine CD8 T Cell Responses.
| dc.contributor.author | Tinega, A. N. | |
| dc.contributor.author | Pellé, R. | |
| dc.contributor.author | Kang'a, S. | |
| dc.contributor.author | Gicheru, M. M. | |
| dc.contributor.author | Taracha, E. | |
| dc.contributor.author | Nene, V. | |
| dc.contributor.author | Graham, S. P. | |
| dc.date.accessioned | 2014-01-14T15:08:22Z | |
| dc.date.available | 2014-01-14T15:08:22Z | |
| dc.date.issued | 2009 | |
| dc.description | doi: 10.1016/j.vetimm.2009.01.008. | en_US |
| dc.description.abstract | Immunity to the bovine apicomplexan parasite Theileria parva is associated with MHC-I restricted CD8+ T cell responses directed against the intralymphocytic schizont stage of the parasite. A number of schizont-stage antigens that are targets of CD8+ T cell responses from immune animals have been identified but an effective delivery strategy that consistently induces protective CD8+ T cell responses remains to be developed. This study aimed to determine whether fusing Tat, a cell penetrating peptide (CPP) from HIV-1 TAT, to a CD8+ T cell target antigen from T. parva (Tp2) enhances the cytosolic delivery and subsequent stimulation of bovine CD8+ T cell responses in vitro. Using IFN-gamma ELISpot and cytotoxicity assays, it was demonstrated that recombinant Tat-Tp2 fusion protein possessed a superior ability to access MHC-I processing and presentation pathway and to stimulate CD8+ T cell responses compared to recombinant Tp2 protein. Exposure of APC to Tat-Tp2 protein for only 30 min was sufficient for protein uptake and stimulation of CD8+ T cells. This work describes for the first time the utility of a CPP to enhance MHC-I presentation in a veterinary species and supports the evaluation of CPP fusion proteins in the induction of CD8+ T cell responses in vivo. | en_US |
| dc.identifier.uri | http://ir-library.ku.ac.ke/handle/123456789/8547 | |
| dc.language.iso | en | en_US |
| dc.publisher | PubMed | en_US |
| dc.title | Fusion of a Cell Penetrating Peptide from HIV-1 TAT to the Theileria Parva Antigen Tp2 Enhances the Stimulation of Bovine CD8 T Cell Responses. | en_US |
| dc.type | Article | en_US |