Isolation of high quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis)
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Date
2009-07
Authors
Muge, E.
Burg, K.
Kadu, C.
Muchugi, Alice Njeri
Lemurt, S.
Jamnadass, R.
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
The genus Warburgia belongs to the Canellaceae, a small family of tropical aromatic tree species important for their antibacterial and antifungal medicinal qualities amongst many other functions. Many published protocols for nucleic acid isolation failed to yield sufficiently good quality amounts for analysis. We have therefore developed a simple and fast CTAB (cetyl-trimethyl ammonium bromide)-based extraction protocol for routine isolation of high-quality nucleic acids. Good quality genomic DNA (gDNA) and RNA was obtained and was suitable for PCR amplification, restriction endonuclease digestion, Southern blotting and RT-PCR, respectively.
Description
Keywords
Nucleic acid extraction, polyphenols, polysaccharides, medicinal plants.
Citation
African Journal of Biotechnology Vol. 8 (13), pp. 3036-3040