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dc.contributor.authorMuge, E.
dc.contributor.authorBurg, K.
dc.contributor.authorKadu, C.
dc.contributor.authorMuchugi, Alice Njeri
dc.contributor.authorLemurt, S.
dc.contributor.authorJamnadass, R.
dc.date.accessioned2013-01-23T12:41:05Z
dc.date.available2013-01-23T12:41:05Z
dc.date.issued2009-07
dc.identifier.citationAfrican Journal of Biotechnology Vol. 8 (13), pp. 3036-3040en_US
dc.identifier.issn1684-5315
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/6285
dc.description.abstractThe genus Warburgia belongs to the Canellaceae, a small family of tropical aromatic tree species important for their antibacterial and antifungal medicinal qualities amongst many other functions. Many published protocols for nucleic acid isolation failed to yield sufficiently good quality amounts for analysis. We have therefore developed a simple and fast CTAB (cetyl-trimethyl ammonium bromide)-based extraction protocol for routine isolation of high-quality nucleic acids. Good quality genomic DNA (gDNA) and RNA was obtained and was suitable for PCR amplification, restriction endonuclease digestion, Southern blotting and RT-PCR, respectively.en_US
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectNucleic acid extractionen_US
dc.subjectpolyphenolsen_US
dc.subjectpolysaccharidesen_US
dc.subjectmedicinal plants.en_US
dc.titleIsolation of high quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis)en_US
dc.typeArticleen_US


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