Isolation of high quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis)

dc.contributor.author	Muge, E.
dc.contributor.author	Burg, K.
dc.contributor.author	Kadu, C.
dc.contributor.author	Muchugi, Alice Njeri
dc.contributor.author	Lemurt, S.
dc.contributor.author	Jamnadass, R.
dc.date.accessioned	2013-01-23T12:41:05Z
dc.date.available	2013-01-23T12:41:05Z
dc.date.issued	2009-07
dc.description.abstract	The genus Warburgia belongs to the Canellaceae, a small family of tropical aromatic tree species important for their antibacterial and antifungal medicinal qualities amongst many other functions. Many published protocols for nucleic acid isolation failed to yield sufficiently good quality amounts for analysis. We have therefore developed a simple and fast CTAB (cetyl-trimethyl ammonium bromide)-based extraction protocol for routine isolation of high-quality nucleic acids. Good quality genomic DNA (gDNA) and RNA was obtained and was suitable for PCR amplification, restriction endonuclease digestion, Southern blotting and RT-PCR, respectively.	en_US
dc.identifier.citation	African Journal of Biotechnology Vol. 8 (13), pp. 3036-3040	en_US
dc.identifier.issn	1684-5315
dc.identifier.uri	http://ir-library.ku.ac.ke/handle/123456789/6285
dc.language.iso	en	en_US
dc.publisher	Academic Journals	en_US
dc.subject	Nucleic acid extraction	en_US
dc.subject	polyphenols	en_US
dc.subject	polysaccharides	en_US
dc.subject	medicinal plants.	en_US
dc.title	Isolation of high quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis)	en_US
dc.type	Article	en_US

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