Production and Characterization of Cellulolytic Enzymes by Chaetomium Globosum for Biomass Saccharification and Ethanol Production
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Date
2024
Authors
Munyasi, Kelvin Masinde
Omwenga, George Isanda
Mwamburi, Fredrick Mjomba
Journal Title
Journal ISSN
Volume Title
Publisher
Discovery Journals
Abstract
This study aimed to isolate, identify and characterize cellulase-producing fungi from a decaying tree trunk, and determine the effects of incubation time, moisture content and initial medium pH on cellulase production using untreated maize cobs and sugarcane bagasse under solid state fermentation after that, to saccharify for ethanol production. Samples were collected from Ngong Forest, Kenya, and screened for the isolation of cellulase producing fungi. The isolated fungi were grown to obtain pure cultures before DNA extraction, PCR amplification and sequencing. The fungus was cultured on substrates for cellulase production with enzymes obtained after fermentation subjected to cellulase assays; filter paper, exoglucanase and endoglucanase. Molecular data analysis was performed using the NCBI-BLAST algorithm and MEGA 11.0 software to identify the isolated fungus, while enzyme activity analysis was done using one-way ANOVA with R software at the P≤0.05 significance level, and the significant differences were determined via the Tukey post hoc test. The isolated fungus was identified as Chaetomium globosum. For the effect of incubation time on cellulase production, the fungus exhibited high enzyme production on different days within the incubation period, and the highest cellulase activity was recorded at a moisture content ratio of 1:2 and initial medium pH of 5. Saccharification optimization studies showed a 7% (v/v) enzyme concentration, 12% (v/w) substrate concentration and hydrolysis time of 72 hours were optimal for the maximum yield of reducing sugars. The total reducing sugar produced maximum bioethanol yield at 72 hours when Saccharomyces cerevisiae was used as a fermentation agent.
Description
Article
Keywords
Fungus, Cellulase, Enzymes, Production, Incubation, Moisture content, pH, Chaetomium globosum, Substrate, Reducing sugar, Fermentation, Ethanol
Citation
Munyasi, K. M., Omwenga, G. I., & Mwamburi, F. M. (2024). Production and characterization of cellulolytic enzymes by chaetomium globosum for biomass saccharification and ethanol production.