Analysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphism
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Date
2016
Authors
Gacheri, Nkatha
Wanjala, Bramwel W.
Jamnadass, Ramni
Muchugi, Alice
Journal Title
Journal ISSN
Volume Title
Publisher
Academic Journals
Abstract
Warburgia ugandensis Sprague (Canellaceae) occurs in East and Central Africa and is an important
multipurpose tree species. Over-exploitation of natural forests for medicinal purposes and clearance for
farming threaten the species survival. Cultivation of the tree species would ensure sustainable
medicinal source and its conservation. However, on-farm genetic diversity of the species is currently
unknown. The genetic diversity of the on-farm W. ugandensis populations and their proximate natural
populations were analyzed using the amplified fragment length polymorphism (AFLP). Four primer
combinations produced a total of 223 polymorphic bands. Both the natural and on-farm populations
had high genetic diversity ranging from H = 0.2892 to H = 0.1278. Principal co-ordinates analysis and
dendrogram separated the ten populations into two major groups corresponding to Kenyan and
Tanzanian populations, respectively. Ugandan populations were shared between the two major groups;
this is probably because Uganda is believed to be the centre of diversity for W. ugandensis. Close
genetic relationships between the on-farm and their proximate natural population were revealed.
Analysis of molecular variance (AMOVA) revealed that a total of 54% AFLP variation resided within
populations with 46% reside among populations. The high genetic diversity of W. ugandensis on-farm
populations could be useful in germplasm collection and conservation strategies.
Description
DOI: 10.5897/AJB2016.15282
Keywords
Warburgia ugandensis, Amplified fragment length polymorphism (AFLP), Domestication, Genetic diversity, On-farm, Natural
Citation
African Journal of Biotechnology Vol. 15(31), pp. 1673-1680, 3 August, 2016