Analysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphism

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dc.contributor.authorGacheri, Nkatha
dc.contributor.authorWanjala, Bramwel W.
dc.contributor.authorJamnadass, Ramni
dc.contributor.authorMuchugi, Alice
dc.date.accessioned2017-11-27T08:32:49Z
dc.date.available2017-11-27T08:32:49Z
dc.date.issued2016
dc.descriptionDOI: 10.5897/AJB2016.15282en_US
dc.description.abstractWarburgia ugandensis Sprague (Canellaceae) occurs in East and Central Africa and is an important multipurpose tree species. Over-exploitation of natural forests for medicinal purposes and clearance for farming threaten the species survival. Cultivation of the tree species would ensure sustainable medicinal source and its conservation. However, on-farm genetic diversity of the species is currently unknown. The genetic diversity of the on-farm W. ugandensis populations and their proximate natural populations were analyzed using the amplified fragment length polymorphism (AFLP). Four primer combinations produced a total of 223 polymorphic bands. Both the natural and on-farm populations had high genetic diversity ranging from H = 0.2892 to H = 0.1278. Principal co-ordinates analysis and dendrogram separated the ten populations into two major groups corresponding to Kenyan and Tanzanian populations, respectively. Ugandan populations were shared between the two major groups; this is probably because Uganda is believed to be the centre of diversity for W. ugandensis. Close genetic relationships between the on-farm and their proximate natural population were revealed. Analysis of molecular variance (AMOVA) revealed that a total of 54% AFLP variation resided within populations with 46% reside among populations. The high genetic diversity of W. ugandensis on-farm populations could be useful in germplasm collection and conservation strategies.en_US
dc.identifier.citationAfrican Journal of Biotechnology Vol. 15(31), pp. 1673-1680, 3 August, 2016en_US
dc.identifier.issn1684-5315
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/17859
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectWarburgia ugandensisen_US
dc.subjectAmplified fragment length polymorphism (AFLP)en_US
dc.subjectDomesticationen_US
dc.subjectGenetic diversityen_US
dc.subjectOn-farmen_US
dc.subjectNaturalen_US
dc.titleAnalysis of the impact of domestication of Warburgia ugandensis (Sprague) on its genetic diversity based on amplified fragment length polymorphismen_US
dc.typeArticleen_US
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