RP-Department of Biochemistry and Biotechnology

Permanent URI for this collection

https://repository.ku.ac.ke/handle/123456789/1380

Browse

Browsing RP-Department of Biochemistry and Biotechnology by Subject "2, 4-dichlorophexyacetic acid"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    In vitro regeneration of selected commercial Tanzanian open pollinated maize varieties
    (Academic Journals, 2012-03) Seth, Miccah Songelael; Bedada, Leta Tulu; Mneney, Emmarold E.; Oduor, R.O.; Machuka, Jesse
    Four Tanzanian open pollinated maize varieties namely; Kito, Situka M-1, Staha and TMV-1 were regenerated in vitro using immature zygotic embryos as ex-plants. Callus induction was achieved using Murashige and Skoog (MS) basal medium supplemented with 1, 1.5, 2 or 2.5 mg/l of 2, 4-D. Callus induction was significantly affected by the genotype of the varieties. Among the varieties tested, Kito, Situka M-1 and Staha had significantly higher callus induction frequency of 84.4, 92.7 and 88.7%, respectively compared to TMV-1 (43.3%). Embryogenic callus induction percentage was significantly influenced by the genotype, 2, 4-D concentrations and their interaction effect implying differential response of the genotypes to 2, 4-D concentrations. Among the genotypes tested, Kito gave significantly (p<0.05) the highest embryogenic callus frequency of 69.5%. Great variations were observed among the genotypes with regard to regeneration frequencies. Staha was comparatively the most regenerable variety with RF of 32.6% than even Kito which had the highest callus induction and embryogenic callus formation. MS medium amended with 1 mg/l 2, 4-D was observed to be the optimum formulation for the induction of embryogenic calli for genotypes tested in this study. Using this regeneration system Situka M-1, Staha and TMV-1 can now be improved against various production constraints through genetic engineering.