Genotyping of Kenyan Passiflora Edulis Flavicarpa Hybrid Accessions and their Parents using SSR Markers
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Date
2016
Authors
Matheri, F.
Teya, F.
Kioko, F.
Mawia, A.M.
Mwangi, M.
Kirubi, D.T.
Ngugi, M.
Runo, Steven
Journal Title
Journal ISSN
Volume Title
Publisher
Society for Plant Research
Abstract
The Passion fruit is a significant income earner for Kenya, ranking
third after avocado and mango in terms of foreign exchange earnings.
Increased research activities targeting variety improvement and
development of new varieties have led to development of new
passion fruit varieties by KALRO. These varieties are KPF-4,
KPF-11 and KPF-12 which are hybrids of natural crosses of the
coastal yellow varieties and purple variety. Despite these gains
in breeding, there is little information on molecular variability of
the hybrids as well as the parents. The present study aimed at
evaluating the genetic variation of the hybrid and parent varieties
using SSR markers. DNA was extracted using a modified CTAB
protocol followed by PCR and analysis done using DARWIN 6 and
GenAlex 6.502 software. The resulting dissimilarity matrix showed
existing genetic variability among accessions within and among
the varieties studied. Further, the results of principal component
analysis and phylogenetic tree showed genetic homogeneity within
the assigned populations. The findings of this study will supplement
the existing body of knowledge in passion fruit breeding.
Description
DOI: 10.4172/2229-4473.1000181
Keywords
Hybrids, Parents, SSR markers, Variability
Citation
Matheri F, Teya F, Kioko F, Mawia AM, Mwangi M, et al. (2016) Genotyping of Kenyan Passiflora Edulis Flavicarpa Hybrid Accessions and their Parents using SSR Markers. Vegetos 29:4.