Genetic Diversity Studies on Selected Rice (Oryza sativa L) Populations Based on Aroma and Cooked Kernel Elongation

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dc.contributor.authorWambua, F. Kioko
dc.contributor.authorMawia, A. Musyoki
dc.contributor.authorNgugi, M. Piero
dc.contributor.authorKarau, G. Muriira
dc.contributor.authorNyamai, D. Wavinya
dc.contributor.authorLagat, Rose
dc.contributor.authorMatheri, Felix
dc.contributor.authorNgari, L. Ngithi
dc.date.accessioned2015-11-10T08:14:32Z
dc.date.available2015-11-10T08:14:32Z
dc.date.issued2015
dc.descriptionDOI:10.4172/2329-9002.1000158en_US
dc.description.abstractRice (Oryza sativa L.) is the main staple food for more than half of the world’s population. Improving cooking and eating quality of rice is one of the important objectives of many plant breeding programs. Aroma and cooked kernel elongation are two critical parameters that determine the market value, cooking and eating qualities of rice. The objective of this study was to evaluate the genetic diversity of thirteen (13) Oryza sativa L. populations from Kenyan and Tanzanian. Genetic diversity was determined using 8 simple sequence repeats (SSR) markers. Diversity data was analyzed using POWERMARKER version 3.25 and GENALEX v 6.5 software packages. The number of alleles per locus ranged from 2 to 4 alleles with an average of 3.12 across 8 loci. The polymorphic information content (pic) ranged from 0.2920 (RM 282) to 0.6409 (RM 339) in all loci with an average of 0.4821. Pair-wise genetic dissimilarity coefficients ranged from 0.1125 to 0.9003 with an average of 0.5312. The average gene diversity over all SSR loci for the 13 rice varieties was 0.6036, ranging from 0.3550 to 0.6391. Maximum genetic similarity was observed between Kilombero and Supa, BS 370 and BS 217. Minimum genetic similarity was observed between Kahogo and BS 217. Cluster analysis was used to group varieties by constructing dendrograms based on SSR data and morphological characterization of grains. The dendrogram based on SSR data formed two distinct clusters of the 13 rice varieties. RM 339 and RM 241 were the most informative markers and could be used for differentiating rice varieties from diverse geographical origins. Results obtained from this study demonstrated that use of trait specific SSR markers can be relied upon in diversity studies among diverse and closely related genotypes. RM 339 and RM 241 markers are recommended for use in diversity studies and in quality assurance for grading of rice varieties. Further analysis should be carried out using a larger number of samples and markers to come up a more conclusive report on the discriminating power of microsatellite markers based on rice grain quality traits.en_US
dc.identifier.citationJ Phylogen Evolution Biol Volume 3 Issue 4; 2015en_US
dc.identifier.issn2329-9002
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/13848
dc.language.isoenen_US
dc.publisherOMICS Internationalen_US
dc.subjectRiceen_US
dc.subjectAromaen_US
dc.subjectKernel elongationen_US
dc.subjectGenetic diversityen_US
dc.titleGenetic Diversity Studies on Selected Rice (Oryza sativa L) Populations Based on Aroma and Cooked Kernel Elongationen_US
dc.typeArticleen_US
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