Hemoglobin Precipitation: An Index of In Vitro Vasoconstrictive Activities of Methanol Leaf Extracts of Croton Megalocarpus Hutch and Lantana Camara Linn
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Date
2021
Authors
Muindi, Hezron Mutisya
Kibiti, Cromwell Mwiti
Ngugi, Mathew Piero
Journal Title
Journal ISSN
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Publisher
Hindawi
Abstract
(e function of innate hemostasis aids the body in bleeding control, preventing the loss of excessive amounts of blood following
low-degree injuries. However, injuries of a higher degree may require extrinsic intervention to stop life-threatening blood loss.
Astringent agents’ actions result in mechanical constriction of small blood vessels and shrinkage of body tissues, thereby stopping
blood loss. (is enhances the primary phase of hemostasis, where vasoconstriction is the main mechanism at play during the
initial response to injury. (e effects of plant extracts on protein precipitation have been linked to blood vessel vasoconstriction.
Traditionally, the leaves of Croton megalocarpus Hutch and Lantana camara Linn plants are used by communities living in
Makueni County, Kenya, for peripheral bleeding control. However, the effects of extracts of both plants on hemoglobin precipitation have not been evaluated scientifically. In the current study, the activities of methanol extracts of C. megalocarpus (H.)
and L. camara (L.) on blood protein precipitation were investigated. (e leaves were harvested, cleaned, air-dried, milled, and
extracted in absolute methanol before being concentrated into dry powders. A qualitative phytochemical screen revealed the
presence of terpenoids, steroids, tannins, phenols, flavonoids, reducing sugars, cardiac glycosides, and carbohydrates in the
methanol extract of C. megalocarpus (H.). (e methanol extracts of L. camara (L.) contained cardiac glycosides, saponins, tannins,
phenols, terpenoids, reducing sugars, and carbohydrates. (e hemoglobin precipitation ability of various concentrations of
extracts using mice samples was presented as relative astringency following the tannic acid external standard method. Methanol
extracts C. megalocarpus (H.) and L. camara (L.) had significantly higher relative astringency compared with the normal control,
indicating a protein precipitating activity. (e relative astringency observed in both plant extracts is linked to the activity of
tannins, phenols, flavonoids, and saponins detected during preliminary phytochemical screening
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Citation
Muindi, H. M., Kibiti, C. M., & Ngugi, M. P. (2021). Hemoglobin Precipitation: An Index of In Vitro Vasoconstrictive Activities of Methanol Leaf Extracts of Croton megalocarpus Hutch and Lantana camara Linn. Evidence-Based Complementary and Alternative Medicine, 2021.