Resolving Intergenotypic Striga Resistance in Sorghum

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dc.contributor.authorMutinda, Sylvia
dc.contributor.authorMobegi, Fredrick M.
dc.contributor.authorHale, Brett
dc.contributor.authorDayou, Olivier
dc.contributor.authorAteka, Elijah
dc.contributor.authorWijeratne, Asela
dc.contributor.authorWicke, Susann
dc.contributor.authorBellis, Emily S.
dc.contributor.authorRuno, Steven
dc.date.accessioned2023-10-26T12:47:29Z
dc.date.available2023-10-26T12:47:29Z
dc.date.issued2023-09
dc.descriptionArticleen_US
dc.description.abstractGenetic underpinnings of host–pathogen interactions in the parasitic plant Striga hermonthica, a root parasitic plant that ravages cereals in sub-Saharan Africa, are unclear. We performed a comparative transcriptome study on five genotypes of sorghum exhibiting diverse resistance responses to S. hermonthica using weighted gene co-expression network analysis (WGCNA). We found that S. hermonthica elicits both basal and effector-triggered immunity—like a bona fide pathogen. The resistance response was genotype specific. Some resistance responses followed the salicylic acid-dependent signaling pathway for systemic acquired resistance characterized by cell wall reinforcements, lignification, and callose deposition, while in others the WRKY-dependent signaling pathway was activated, leading to a hypersensitive response. In some genotypes, both modes of resistance were activated, while in others either mode dominated the resistance response. Cell wall-based resistance was common to all sorghum genotypes but strongest in IS2814, while a hypersensitive response was specific to N13, IS9830, and IS41724. WGCNA further allowed for pinpointing of S. hermonthica resistance causative genes in sorghum, including glucan synthase-like 10 gene, a pathogenesis-related thaumatin-like family gene, and a phosphoinositide phosphatase gene. Such candidate genes will form a good basis for subsequent functional validation and possibly future resistance breeding.en_US
dc.description.sponsorshipNational Academies of Science USAID Arkansas Biosciences Institute African Union Commission German Academic Exchange Service— Deutscher Akademischer Austauschdienst (DAAD)en_US
dc.identifier.citationMutinda, S., Mobegi, F. M., Hale, B., Dayou, O., Ateka, E., Wijeratne, A., ... & Runo, S. (2023). Resolving intergenotypic Striga resistance in sorghum. Journal of Experimental Botany, 74(17), 5294-5306.en_US
dc.identifier.urihttps://doi.org/10.1093/jxb/erad210
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/27080
dc.language.isoenen_US
dc.publisherJournal of Experimental Botanyen_US
dc.subjectCell wall-based resistanceen_US
dc.subjectcomparative transcriptomicsen_US
dc.subjectlignin-based resistanceen_US
dc.subjectparasitic plantsen_US
dc.subjectpathogenassociated molecular patternsen_US
dc.subjectprogrammed cell deathen_US
dc.subjectweighted gene co-expression networksen_US
dc.titleResolving Intergenotypic Striga Resistance in Sorghumen_US
dc.typeArticleen_US
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