Mouse cytokine profile skewed towards Th2 in pregnancy during infection with Brucella abortus S19 strain

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dc.contributor.authorNgeranwa, J.J.N.
dc.contributor.authorWamonje, F. O.
dc.contributor.authorWaihenya, R. K.
dc.contributor.authorNg'ang'a, Z.
dc.date.accessioned2014-07-21T14:49:28Z
dc.date.available2014-07-21T14:49:28Z
dc.date.issued2011
dc.descriptionhttp://dx.doi.org/10.4314/thrb.v13i2.54753en_US
dc.description.abstractThe two classes of cytokines Th1 and Th2 determine the type of immune response elicited. The Th2 immune response is associated with successful pregnancy. Brucellosis is an intracellular bacterium that elicits the Th1 response and is known to cause spontaneous abortion in mammalian species. This study sought to determine if Brucella infection causes spontaneous abortion by causing the circulating cytokine profile be Th1 dominant during pregnancy. Forty-eight Swiss white mice were used in this murine model and the S19 strain of Brucella abortus was used in as the infective agent. Pregnant mice in the test group were injected intraperitoneally with 105-8 CFU of Brucella and cytokine profile evaluated over the three trimesters of pregnancy. Pregnant mice in the control group were left to go through normal pregnancy and their cytokine profile evaluated over the three trimesters of pregnancy. Cytokines in serum samples were analyzed by Cytometric Bead Array. The data was analyzed using the Paired T- test and P<0.05 was considered significant. IFN-γ and TNF-α represented the Th1 cytokines while IL-4 and IL-5 represented the Th2 cytokines. None of the mice in the test group had spontaneous abortion. IFN-γ and TNF-α had no significant differences between cytokine levels for infected and uninfected groups in all 3 trimesters of pregnancy. IL-4 levels had significant differences in all three trimesters of pregnancy (t= 13, P= 0.036, 0.0071 and 0.0277). IL-5 levels had significant differences second trimester (t=14, P= 0.0075). The cytokine profile was robustly Th2. In conclusion, Brucella abortus cannot cause spontaneous abortion by altering the mouse cytokine profile towards ThI in pregnancy. Elevated IL-4 levels with corresponding suppression of IFN-γ can be used as a marker for successful pregnancy in brucellosis.en_US
dc.identifier.citationTanzania Journal of Health Research Vol 13, No 2 (2011)en_US
dc.identifier.issn0856-6496
dc.identifier.other1821-9241
dc.identifier.urihttp://www.ajol.info/index.php/thrb/article/view/54753/57172
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/10525
dc.language.isoenen_US
dc.subjectCytokinesen_US
dc.subjectBrucellaen_US
dc.subjectInterleukinen_US
dc.subjectInterferonen_US
dc.subjectT - helper 1en_US
dc.subjectT - helper 2en_US
dc.titleMouse cytokine profile skewed towards Th2 in pregnancy during infection with Brucella abortus S19 strainen_US
dc.typeArticleen_US
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