Micropropagation of an Endangered Medicinal and Indigenous Multipurpose Tree Species: Erythrina abyssinica

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dc.contributor.authorKirika, Margaret W.
dc.contributor.authorKahia, Jane W.
dc.contributor.authorDiby, Lucien N.
dc.contributor.authorNjagi, E.N.M.
dc.contributor.authorDadjo, Colombe
dc.contributor.authorKouame, Christophe
dc.date.accessioned2016-01-13T12:25:14Z
dc.date.available2016-01-13T12:25:14Z
dc.date.issued2015
dc.descriptionResearch Articleen_US
dc.description.abstractErythrina abyssinica (E. abyssinica) is a multipurpose tree and a well-known medicinal plant which is conventionally propagated mainly by seeds. This method may produce a high degree of genetic variability and consequently decrease the medicinal value of the plant. Besides, the seeds have low germination rate and propagation is restricted to rainy season. Hence, there is need to develop a propagation protocol which produces a uniform plants and one which is not restricted to seasons. The objective of this study was to establish an in vitro propagation protocol for the multiplication of E. abyssinica. Seeds were sterilized and germinated in vitro to get sterile starting explants. Sterilization of the seeds was evaluated using different concentrations of a commercial bleach (JIK) ranging from 10%, 20% to 30% for 25 minutes. Kinetin (2.25, 4.50, 6.75, and 9.0 mg/L), and benzylaminopurine (BAP; 2.15, 4.30, 6.46, and 8.61 mg/L) were evaluated in separate experiments for their effect to induce microshoots from nodal explants. Rooting of the microshoot was carried out using half strength Murashige and Skoog (MS) media supplemented with indolebutyric acid (IBA) (0.20, 0.51, and 1.02 mg/L). Statistical analysis software (SAS) package was used to perform analysis of variance on the data to test the significance of the difference between treatments. The result of the sterilization experiment indicated that 10% JIK gave the highest percentage (55%) of clean seeds. Benzylaminopurine evaluated at 8.61 mg/L gave the highest mean number of microshoots (6.80 ± 1.24) after 28 days. On the other hand, IBA evaluated at 0.51mg/L gave the highest mean root length (6.00 ± 01.85 cm). The regenerated plants were acclimatized in the greenhouse and 65% survival rate was recorded after one month. With the increasing worldwide demand for medicinal plants as an alternative to prescription drugs, ex situ, in situ conservation programs and true to type mass propagation of E. abyssinica could benefit from the findings of this study. This is the first report on micropropagation of E. abyssinica.en_US
dc.identifier.citationHorticultural Science 50(5):738–743. 2015.en_US
dc.identifier.issn0862-867X
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/14032
dc.language.isoenen_US
dc.publisherCzech Academy of Agricultural Sciencesen_US
dc.subjectErythrina abyssinicaen_US
dc.subjectMicroshooten_US
dc.subjectMedicinalen_US
dc.subjectNodal explanten_US
dc.titleMicropropagation of an Endangered Medicinal and Indigenous Multipurpose Tree Species: Erythrina abyssinicaen_US
dc.typeArticleen_US
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