Antinociceptive and antiiflammatory potential of dichloromethane: methanolic leaf extracts of caesalpinia volkensii and maytenus obscura in animal models
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Date
2015-09
Authors
Maina, Mwangi B.
Maina, G. S.
Muriithi, Njagi J.
Kiambi, M. J.
Umar, Aliyu
Njoroge, A. W.
Mwonjoria, K. J.
Ngugi, Piero M.
Mburu, David N.
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Abstract
Caesalpinia volkensii and Maytenus obscura are
specialised plants that grow in Mbeere County of
Eastern region of Kenya. Species belonging to genus
maytenus are widely used in folk medicine such as
antiseptic, antiasthmatic, fertility-regulating agents,
antitumor and antiulcer. The leaf decoction of
Caesalpinia volkensii is taken to fight pains during
pregnancy. Pregnant women take powdered pods
dissolved in water to relieve stomach-ache. They are
also used to treat gonorrhoea and bilharzia. Seeds
are used to cure stomach ulcers. Flower buds are
crushed and applied to the eye to treat eye problems.
This study is designed to evaluate the antinociceptive
and anti-inflammatory activity of Caesalpinia volkensii
and Maytenus obscura plants. In order to assess the
antinociceptive and anti-inflammatory effects, formalin
induced writhing response model and carrageenan
induced paw edema model will be used in and Wister
albino rats and mice. In both cases, leaves extract will
be administered (2gm/kg body weight) and the
obtained effects will be compared with commercially
available antinociceptive and anti-inflammatory drug
Diclofenac sodium (15mg/kg body weight). DMSO
(2ml/kg body weight) will be used as a control for the
study. The plant sample of the leaves will be dried at
room temperature under shade for one month and
ground into powder. Two hundred grams of the
powdered leaves material will be soaked to DCM and
ethanol in the ratio of 1:1 for 3 h. The extract will be
filtered and concentrated using a rotator evaporator.
The experimental rats and mice will be divided into
four groups; normal group, diseased, control and
experimental group. Pain will be induced using
formalin while inflammation will be induced using
carrageenan. The experimental group will be treated
with predetermined quantities of extracts of both plant
leaves. The dichloromethane:methanolic extract of
leaves of the plant will be evaluated for significant
antiinflammation and antinociceptive activities using
several experimental models in rats and mice when
compared with the standard conventional drugs. The
experimental data will be analyzed using paired t-test
and analysis of variance (ANOVA). Should the
claimed effectiveness be validated by this study, the
plant will be recommended for further studies as a bio
source into producing standardized herbal formulation
more effective in the treatment of pain and
inflammation with less toxic and less costly than
current synthetic drugs.
Description
DOI: 10.13140/RG.2.1.2202.7761
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Citation
https://www.researchgate.net/publication/304989243; DOI: 10.13140/RG.2.1.2202.7761