Identification of root-knot nematode species occurring on tomatoes in Kenya: use of isozyme phenotypes and PCR-RFLP

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dc.contributor.authorWaceke, J.W.
dc.contributor.authorBirithia, R.
dc.contributor.authorLomo, P.
dc.contributor.authorMasiga, D.
dc.date.accessioned2013-12-09T13:13:44Z
dc.date.available2013-12-09T13:13:44Z
dc.date.issued2012
dc.descriptiondoi:10.1017/S1742758412000173en_US
dc.description.abstractRoot-knot nematodes (RKN) are serious pests of tomato production in Kenya. Accurate identification of the plant parasitic nematodes is important for their effective management. A study conducted to assess their prevalence and identify RKN occurring on tomatoes in Central Kenya showed infestation of the crop in all the three districts. Of the total sampled plants (N¼900), the RKN infestation level ranged from 28 to 62%. RKN disease severity ranged from 2.5 to 5.3 in all the locations. Meloidogyne incognita,M. javanica and M. arenaria were the only species found infesting tomatoes in these areas. Female nematodes sampled from the symptomatic root system that had root galls characteristic of RKN were analysed by isozyme phenotypes of esterase (EST) and malate dehydrogenase (MDH). EST phenotype was polymorphic and enabled identification of the three different species, while MDH was monomorphic. Polymerase chain reaction-restriction fragment length polymorphism with region between mitochondrial cytochrome oxidase subunit II and large subunit ribosomal RNA (mtDNA COII- LSUrRNA) using HinfI showed that all isolates of M. incognita could be digested into three restriction fragments of about 1300, 400 and 100 bp, except for one species that showed an additional restriction site, giving four fragments of 900, 420, 380 and 100 bp. The 1800 bp PCR product of M. javanica was not digested by HinfI. Meloidogyne arenaria (EST phenotype A1) PCR product was digested into two restriction fragments of about 1700 and 100bp, while M. arenaria (EST phenotype A2) had two restriction fragments at1100 and 100 bp. Key words: Meloidogynespp, tomatoes, isozyme profiles, molecular identificationen_US
dc.identifier.citationInternational Journal of Tropical Insect Science Vol. 32, No. 2, pp. 78–84, 2012en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/7909
dc.language.isoenen_US
dc.publisherInternational Journal of Tropical Insect Scienceen_US
dc.subjectMeloidogynesppen_US
dc.subjecttomatoesen_US
dc.subjectisozyme profilesen_US
dc.subjectmolecular identificationen_US
dc.titleIdentification of root-knot nematode species occurring on tomatoes in Kenya: use of isozyme phenotypes and PCR-RFLPen_US
dc.typeArticleen_US
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