Safety and Efficacy Assessment of Bacteriophages Isolated from Kibera, Kenya Wastewater Plant, Against Multidrug-Resistant Pseudomonas Aeruginosa Infection in BALB/C Mice

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dc.contributor.authorOmwega, Meshack Tweya
dc.contributor.authorMaingi, John M.
dc.contributor.authorNyamache, Anthony Kebira
dc.contributor.authorNyachieo, Atunga
dc.date.accessioned2023-06-16T07:58:38Z
dc.date.available2023-06-16T07:58:38Z
dc.date.issued2021
dc.descriptionresearch articleen_US
dc.description.abstractPseudomonas aeruginosa is a pathogen of great clinical importance to both humans and animals. It causes pneumonia in cystic fibrosis patients, and it is responsible for the infections of blood and lungs during surgery. Increased antibiotic use has led to the emergence of multidrug-resistant strains of P. aeruginosa. Recently, phage therapy has attracted much attention as a promising alternative against the increasing antimicrobial resistance. This study determined the safety and efficacy of phage therapy against virulent P. aeruginosa in a murine model. Phage PaCIKb2 was isolated from sewage water. Morphological characterization by transmission electron microscope was done. We assessed the phages' antimicrobial effect in vitro and in biofilms, its growth kinetics, host ranges, temperature and pH stability. Therapeutic safety and efficacy were observed 24 hours post-infection with virulent P. aeruginosa in a murine model. Transmission Electron Microscopy revealed phage PaCIKb2 to belong to the family myoviridae. The phage was found to be high temperature tolerant (up to 50°C). It was active between pH ranges (5 and 11), had a latent period of 15 minutes with a burst size of 316 viral particles, and exhibited a narrow host range. After intravenous phage administration dose (2×109 PFU/ml) post-infection with virulent P. aeruginosa, the presence of phages in vivo and reduction of bacterial loads in mice was observed. A reduction in mice tissue inflammation suggested the effectiveness of phage PaCIKb2 phage therapy. This research gives data that supports the use of phage therapy against multidrug-resistant P. aeruginosa.en_US
dc.identifier.citationMeshack Tweya Omwega, John M. Maingi, Anthony Kebira Nyamache, and Atunga Nyachieo,, “Safety and Efficacy Assessment of Bacteriophages Isolated from Kibera, Kenya Wastewater Plant, against Multidrug-Resistant Pseudomonas aeruginosa Infection in BALB/c Mice.” American Journal of Infectious Diseases and Microbiology, vol. 10, no. 1 (2022): 11-21. doi: 10.12691/ajidm-10-1-3en_US
dc.identifier.otherDOI:10.12691/ajidm-10-1-3
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/25852
dc.language.isoenen_US
dc.publisherScience and Education Publishingen_US
dc.subjectphage therapyen_US
dc.subjectMDR-PAen_US
dc.subjectBacteriophage (Phage)en_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectantimicrobial resistanceen_US
dc.subjectmulti-drug resistanceen_US
dc.titleSafety and Efficacy Assessment of Bacteriophages Isolated from Kibera, Kenya Wastewater Plant, Against Multidrug-Resistant Pseudomonas Aeruginosa Infection in BALB/C Miceen_US
dc.typeArticleen_US
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