Conducting polyamic acid membranes for sensing and site-directed immobilization of proteins

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dc.contributor.authorNoah, Naumih M
dc.contributor.authorOmowunmi, A. Sadik
dc.date.accessioned2015-03-25T07:58:43Z
dc.date.available2015-03-25T07:58:43Z
dc.date.issued2013
dc.description4th International Conference and Exhibition on Analytical & Bioanalytical Techniques October 15-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USAen_US
dc.description.abstractPoly (amic) acid (PAA) is a functionalized conducting polymer substrate that provides electrochemical detection control of biospecific binding. In this work, we report a biosensor platform based on the PAA for oriented immobilization of biomolecules. The PAA was used to covalently attach biomolecules, resulting in a significant improvement in the detection sensitivity. The biosensor sensing elements comprise a layer of PAA antibody (or antigen) composite self assembled onto gold (Au) electrode via N-hydroxysuccinimide (NHS) and 1 ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) linking. The modified PAA was characterized by Fourier transform infrared (FTIR), 1H nuclear magnetic resonance (NMR), and electrochemical techniques. Cyclic voltammetry and impedance spectroscopy experiments conducted on electrodeposited PAA on Au electrode using ferricyanide produced a measurable decrease in the diffusion coefficient compared with the bare electrode, indicating some retardation of electron transfer within the bulk material of the PAA. Thereafter, the modified PAA surface was used to immobilize antibodies and then to detect inducible nitric oxide synthase (a pain biomarker) and mouse immunoglobulin G (IgG) using enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and amperometric techniques. ELISA results indicated a significant amplified signal by the modified PAA, whereas the SPR and amperometric biosensors produced significant responses as the concentration of the antigen was increaseden_US
dc.identifier.citationJ Anal Bioanal Tech 2013, 4:5en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/12415
dc.language.isoenen_US
dc.titleConducting polyamic acid membranes for sensing and site-directed immobilization of proteinsen_US
dc.typeArticleen_US
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