Conducting polyamic acid membranes for sensing and site-directed immobilization of proteins
Loading...
Date
2013
Authors
Noah, Naumih M
Omowunmi, A. Sadik
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Poly (amic) acid (PAA) is a functionalized conducting polymer substrate that provides electrochemical detection control
of biospecific binding. In this work, we report a biosensor platform based on the PAA for oriented immobilization of
biomolecules. The PAA was used to covalently attach biomolecules, resulting in a significant improvement in the detection
sensitivity. The biosensor sensing elements comprise a layer of PAA antibody (or antigen) composite self assembled onto gold (Au)
electrode via N-hydroxysuccinimide (NHS) and 1 ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) linking. The modified
PAA was characterized by Fourier transform infrared (FTIR), 1H nuclear magnetic resonance (NMR), and electrochemical
techniques. Cyclic voltammetry and impedance spectroscopy experiments conducted on electrodeposited PAA on Au electrode
using ferricyanide produced a measurable decrease in the diffusion coefficient compared with the bare electrode, indicating some
retardation of electron transfer within the bulk material of the PAA. Thereafter, the modified PAA surface was used to immobilize
antibodies and then to detect inducible nitric oxide synthase (a pain biomarker) and mouse immunoglobulin G (IgG) using
enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and amperometric techniques. ELISA results
indicated a significant amplified signal by the modified PAA, whereas the SPR and amperometric biosensors produced significant
responses as the concentration of the antigen was increased
Description
4th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
October 15-17, 2013 Hampton Inn Tropicana, Las Vegas, NV, USA
Keywords
Citation
J Anal Bioanal Tech 2013, 4:5