Use of Polyclonal Antibody for the Diagnosis of Human African Trypanosomiasis
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Date
2023
Authors
Oumar, Dawala Koromtili
Munyao, Matthew Mutinda
Mwangi, Anne Wanjiru
Waihenya, Rebecca Wanjiku
Rotich, Peter Kipkemboi
Irekwa, Robinson Mugasiali
Nyandwaro, Tonny Teya
Njoroge, Caroline Wangui
Yego, Joanne Jepkemei
Ndungu, Primrose Muthoni
Journal Title
Journal ISSN
Volume Title
Publisher
SCIRP
Abstract
Human African trypanosomiasis (HAT), commonly known as sleeping sickness is one of the neglected tropical diseases (NTDs), which is fatal if left untreated. Its diagnosis is a challenge since the signs and symptoms of the primary phase are not specific, the existing diagnostic methods have low sensitivity and specificity, and the available drugs have some toxicity. New, robust,
and cost-effective techniques are needed for the early identification of parasites. This study aimed to assess the sensitivity and specificity of two different
types of polyclonal antibodies against T. b. gambiense using antigen detection
ELISA. Polyclonal antibodies against the expressed proteins Tbg I2 and Tbg
I17 were produced using New Zealand white rabbits. The antibody titer measured was greater than 32 g/L after the 3rd immunization for the expressed
protein Tbg I2. For the expressed protein Tbg I17, the antibody titer measured was greater than 32 g/L after the 4th immunization. The sensitivity and
specificity of the Tbg I2 polyclonal antibody confirmed with Polymerase
Chain Reaction (PCR) as gold standard were respectively 89.5% and 80.6%,
while for the Tbg I17 polyclonal antibody, the sensitivity and specificity were
respectively 92.1% and 88.9%. The area under the curve for the Tbg I2 polyclonal antibody was 0.90 ± 0.032, while for the Tbg I17 polyclonal antibody,
the area under the curve was 0.92 ± 0.0. The Tbg I17 polyclonal antibody
produced in New Zealand white rabbits has good sensitivity and good specificity; it can be successfully used in the diagnosis of HAT.
Description
Article
Keywords
Human African Trypanosomiasis, Polyclonal Antibody, Tbg I2 Expressed Protein, Tbg I17 Expressed Protein, Sensitivity, Specificity
Citation
Oumar, D. K., Munyao, M. M., Mwangi, A. W., Waihenya, R. W., Rotich, P. K., Irekwa, R. M., ... & Nzou, S. M. (2023). Use of Polyclonal Antibody for the Diagnosis of Human African Trypanosomiasis. American Journal of Molecular Biology, 13(2), 127-139.