Genetic Transformability of Selected Kenyan Groundnut (Arachis Hypogaea L.) Genotypes with IPT Gene Using Cotyledonary Node Explants
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Date
2022
Authors
Limbua, Purity G
Ngugi, Mathew P
Oduor, Richard O
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Publisher
Hindawi
Abstract
Groundnut (Arachis hypogaea L.) is an important crop in terms of income and nutrition. Despite its importance, groundnut yield
is limited by environmental factors such as drought. (is work reports the genetic transformability of Kenyan groundnut (Arachis
hypogaea L.) genotypes with isopentenyl transferase (IPT) gene towards drought tolerance. (e cotyledonary nodes of six Kenyan
adapted groundnuts genotypes (ICGV 12991, CG 7, Red Valencia, ICGV 90704, Chalimbana, and JL 24) were transformed using
Agrobacterium tumefaciens strain EHA 101 carrying PNOV-IPT binary vector containing an IPTgene, which was driven by SARK
promoter and terminated by TNOS terminator. (e vector also contained the phosphomannose isomerase (PMI) gene for the
selection of transformed tissues. Putative transformants were tested for the presence of the transgene by PCR designed to amplify
the IPT gene sequence. Gene expression was confirmed by RT-PCR. Transformation frequency was calculated as a percentage of
the number of putative transformants divided by the total number of infected cotyledonary nodes. (is ranged from 9.87% for
ICGV 90704 to 19.77% for JL 24. Transformation efficiency was calculated as a percentage of the number of PCR positive plants
divided by the total number of cotyledonary nodes infected. (is ranged from 0% for ICGV 12991 and Chalimbana to 1.74% for JL
24. (e data suggest the possibility of transforming groundnuts with the IPTgene and regenerating normal transgenic plants. (is
information will be useful during the transformation of groundnut towards different factors that affect production.
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Citation
Limbua, P. G., Ngugi, M. P., & Oduor, R. O. (2022). Genetic Transformability of Selected Kenyan Groundnut (Arachis hypogaea L.) Genotypes with IPT Gene Using Cotyledonary Node Explants. Advances in Agriculture, 2022.