Strigolactone Biosynthesis Lgs1 Mutant Alleles Mined from the Sorghum Accession Panel are a Promising Resource of Resistance to Witchweed (Striga) Parasitism

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dc.contributor.authorMutinda, Sylvia
dc.contributor.authorJamil, Muhammad
dc.contributor.authorWang, Jian You
dc.contributor.authorBerqdar, Lamis
dc.contributor.authorAteka, Elijah
dc.contributor.authorBellis, Emily S.
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorRuno, Steven
dc.date.accessioned2023-10-26T12:23:14Z
dc.date.available2023-10-26T12:23:14Z
dc.date.issued2023-09
dc.descriptionArticleen_US
dc.description.abstractSocietal Impact Statement Striga is a parasitic plant that greatly limits the production of Africa's most staple cereals, including sorghum. Infection occurs when the parasite germinates in response to biomolecules emitted into the soil from the host's roots. Some sorghum genotypes harbor a mutation that makes them ineffective in stimulating Striga seed germination. This resistance is of great importance because of its possible application in Striga management. Here, additional resistant sorghum genotypes with varying levels of Striga resistance are discussed in the context of their candidacy for integration in breeding programs and their possible role in alleviating food insecurity in subSaharan Africa by reducing crop losses because of Striga infestation. Summary • Sorghum is a food staple for millions of people in sub-Saharan Africa, but its production is greatly diminished by Striga, a parasitic weed. An efficient and costeffective way of managing Striga in smallholder farms in Africa is to deploy resistant varieties of sorghum. • Here, we leverage genomics and the vast genetic diversity of sorghum— evolutionarily adapted to cope with Striga parasitism in Africa—to identify new Striga-resistant sorghum genotypes by exploiting a resistance mechanism hinged on communication molecules called strigolactones (SLs), exuded by hosts to trigger parasite seed germination. We achieved this by mining for mutant alleles of the LOW GERMINATION STIMULANT 1 (LGS1) that are ineffective in stimulating Striga germination from the sorghum accession panel (SAP). • Our analysis identified lgs1 sorghum genotypes, which we named SAP-lgs1. SAPlgs1 had the SL exudation profile of known lgs1 sorghum, whose hallmark is the production of the low inducer of germination, orobanchol. Laboratory and field resistance screens showed that the SAP-lgs1 genotypes also exhibited remarkable resistance against Striga. • Our findings have the potential to reduce crop losses because of Striga parasitism and therefore have far-reaching implications for improving food security in Africa.en_US
dc.description.sponsorshipRoyal Society Mawazo fellows fund King Abdullah University of Science and Technology Bill and Melinda Gates Foundation African Union Commissionen_US
dc.identifier.citationMutinda, S., Jamil, M., Wang, J. Y., Berqdar, L., Ateka, E., Bellis, E. S., ... & Runo, S. (2023). Strigolactone biosynthesis lgs1 mutant alleles mined from the sorghum accession panel are a promising resource of resistance to witchweed (Striga) parasitism. Plants, People, Planet.en_US
dc.identifier.otherhttps://doi.org/10.1002/ppp3.10442
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/27078
dc.language.isoenen_US
dc.publisherPlants, People, Planeten_US
dc.subjectallele miningen_US
dc.subjectfood securityen_US
dc.subjectLOW GERMINATION STIMULANT 1en_US
dc.subjectStriga resistanceen_US
dc.subjectstrigolactonesen_US
dc.subjectsub-Saharan Africaen_US
dc.subjectwitchweeden_US
dc.titleStrigolactone Biosynthesis Lgs1 Mutant Alleles Mined from the Sorghum Accession Panel are a Promising Resource of Resistance to Witchweed (Striga) Parasitismen_US
dc.typeArticleen_US
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