In vitro regeneration of selected commercial Tanzanian open pollinated maize varieties

Simple item page
dc.contributor.authorSeth, Miccah Songelael
dc.contributor.authorBedada, Leta Tulu
dc.contributor.authorMneney, Emmarold E.
dc.contributor.authorOduor, R.O.
dc.contributor.authorMachuka, Jesse
dc.date.accessioned2012-10-31T11:42:52Z
dc.date.available2012-10-31T11:42:52Z
dc.date.issued2012-03
dc.descriptionPublisher version (Academic Journals Online) available at www.academicjournals.org/ajb/PDF/.../Seth%20et%20al.pdfen_US
dc.description.abstractFour Tanzanian open pollinated maize varieties namely; Kito, Situka M-1, Staha and TMV-1 were regenerated in vitro using immature zygotic embryos as ex-plants. Callus induction was achieved using Murashige and Skoog (MS) basal medium supplemented with 1, 1.5, 2 or 2.5 mg/l of 2, 4-D. Callus induction was significantly affected by the genotype of the varieties. Among the varieties tested, Kito, Situka M-1 and Staha had significantly higher callus induction frequency of 84.4, 92.7 and 88.7%, respectively compared to TMV-1 (43.3%). Embryogenic callus induction percentage was significantly influenced by the genotype, 2, 4-D concentrations and their interaction effect implying differential response of the genotypes to 2, 4-D concentrations. Among the genotypes tested, Kito gave significantly (p<0.05) the highest embryogenic callus frequency of 69.5%. Great variations were observed among the genotypes with regard to regeneration frequencies. Staha was comparatively the most regenerable variety with RF of 32.6% than even Kito which had the highest callus induction and embryogenic callus formation. MS medium amended with 1 mg/l 2, 4-D was observed to be the optimum formulation for the induction of embryogenic calli for genotypes tested in this study. Using this regeneration system Situka M-1, Staha and TMV-1 can now be improved against various production constraints through genetic engineering.en_US
dc.description.sponsorshipThis work constitutes a part of Genetic engineering of maize for drought tolerance project funded by United States Agency for International Development (USAID), and the Multi-Donor Trust Funds (MDTFs), of World Banken_US
dc.identifier.citationAfrican Journal of Biotechnology Vol. 11(22), pp. 6043-6049, 15 March, 2012en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/5889
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subject2, 4-dichlorophexyacetic aciden_US
dc.subjectembryogenic callusen_US
dc.subjectimmature zygotic embryosen_US
dc.subjectregeneration frequencyen_US
dc.subjectsomatic embryosen_US
dc.subjectTanzanian open pollinated maizeen_US
dc.titleIn vitro regeneration of selected commercial Tanzanian open pollinated maize varietiesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Abstract.pdf
Size:
5.47 KB
Format:
Adobe Portable Document Format
Description:
Abstract
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
5.24 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
RP-Department of Biochemistry and Biotechnology