Mediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds

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dc.contributor.authorOchola, Juliet Akoth
dc.contributor.authorCortada, Laura
dc.contributor.authorNg’ang’a, Margaret
dc.contributor.authorHassanali, Ahmed
dc.contributor.authorCoyne, Danny
dc.contributor.authorTorto, Baldwyn
dc.date.accessioned2021-09-20T11:45:59Z
dc.date.available2021-09-20T11:45:59Z
dc.date.issued2020
dc.descriptionA research article published in Frontiers in Plant Scienceen_US
dc.description.abstractPotato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs first need to be stimulated to hatch by chemical components in the host root exudates, yet it remains unknown how most root exudate components influence PCN behavior. Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the root exudate of potato on the hatching response of the PCN, Globodera rostochiensis at varying doses. The eight compounds included the amino acids tyrosine, tryptophan and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine, previously identified in the root exudates of tomato, an alternative host for PCN. In dose-response assays with the individual compounds, the known PCN hatching factors α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47 and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%, respectively, with tomatidine eliciting the lowest hatching response 13%. However, ∼60% of the hatched juveniles failed to emerge from the cyst, which was compoundand concentration-dependent. The amino acids, phytohormones and the negative control (1% DMSO in water), however, were generally non-stimulatory. The use of steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers promise as an environmentally sustainable approach to manage this pest.en_US
dc.identifier.citationOchola, J., Cortada, L., Ng’ang’a, M., Hassanali, A., Coyne, D., & Torto, B. (2020). Mediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds. Frontiers in Plant Science, 11, 649.en_US
dc.identifier.issn1664-462X
dc.identifier.urihttps://www.frontiersin.org/articles/10.3389/fpls.2020.00649/full
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/22531
dc.language.isoenen_US
dc.publisherFrontiersen_US
dc.subjectGlobodera sppen_US
dc.subjectHatching factorsen_US
dc.subjectPotato root exudatesen_US
dc.subjectSemiochemicalsen_US
dc.subjectSteroidal glycoalkaloidsen_US
dc.titleMediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compoundsen_US
dc.typeArticleen_US
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