Mediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds
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Date
2020
Authors
Ochola, Juliet Akoth
Cortada, Laura
Ng’ang’a, Margaret
Hassanali, Ahmed
Coyne, Danny
Torto, Baldwyn
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers
Abstract
Potato (Solanum tuberosum) is a widely consumed staple food crop worldwide whose
production is threatened by potato cyst nematodes (PCN). To infect a host, PCN eggs
first need to be stimulated to hatch by chemical components in the host root exudates,
yet it remains unknown how most root exudate components influence PCN behavior.
Here, we evaluated the influence of eight compounds identified by LC-QqQ-MS in the
root exudate of potato on the hatching response of the PCN, Globodera rostochiensis
at varying doses. The eight compounds included the amino acids tyrosine, tryptophan
and phenylalanine; phytohormones zeatin and methyl dihydrojasmonate; steroidal
glycoalkaloids α-solanine and α-chaconine and the steroidal alkaloid solanidine. We
additionally tested two other Solanaceae steroidal alkaloids, solasodine and tomatidine,
previously identified in the root exudates of tomato, an alternative host for PCN. In
dose-response assays with the individual compounds, the known PCN hatching factors
α-chaconine and α-solanine stimulated the highest number of eggs to hatch, ∼47
and ∼42%, respectively, whereas the steroidal alkaloids (aglycones), solanidine and
solasodine and potato root exudate (PRE) were intermediate, 28% each and 21%,
respectively, with tomatidine eliciting the lowest hatching response 13%. However,
∼60% of the hatched juveniles failed to emerge from the cyst, which was compoundand concentration-dependent. The amino acids, phytohormones and the negative
control (1% DMSO in water), however, were generally non-stimulatory. The use of
steroidal glycoalkaloids and their aglycones in the suicidal hatching of PCN offers
promise as an environmentally sustainable approach to manage this pest.
Description
A research article published in Frontiers in Plant Science
Keywords
Globodera spp, Hatching factors, Potato root exudates, Semiochemicals, Steroidal glycoalkaloids
Citation
Ochola, J., Cortada, L., Ng’ang’a, M., Hassanali, A., Coyne, D., & Torto, B. (2020). Mediation of Potato–Potato Cyst Nematode, G. rostochiensis Interaction by Specific Root Exudate Compounds. Frontiers in Plant Science, 11, 649.