Evaluation of In vivo Toxicity of Methanolic Leaf Extract of Vernonia lasiopus (O. Hoffman)

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dc.contributor.authorMusila, Michael N
dc.contributor.authorMuthoni, Beatrice G
dc.contributor.authorKoech, Samson C
dc.contributor.authorNgugi, Mathew P
dc.contributor.authorMbinda, Wilton M
dc.date.accessioned2021-02-24T13:13:10Z
dc.date.available2021-02-24T13:13:10Z
dc.date.issued2017
dc.descriptionA research article published in Journal of Pharmacognosy & Natural Productsen_US
dc.description.abstractThe main objective of this study was to evaluate the in vivo toxicity of methanolic leaf extract of Vernonia lasiopus. To provide information on the safety of V. lasiopus, we evaluated its acute and sub-chronic toxicity in Wistar rats. For evaluation of acute toxicity of the plant extract, five Wistar rats were orally dosed with 2000 mg/kg body weight sequentially. Sub-chronic toxicity was tested in twenty Wistar rats using three extract doses 100, 300 and 1000 mg/kg body weight. They were orally administered for 28 days. Mortality and toxicity signs were monitored during the study period. At the end of the experiment, the animals were sacrificed, their internal organs weighed and blood samples collected for haematology and biochemical analysis. In acute toxicity, no single death was reported; leading to conclusion that the median lethal dose (LD50) of methanolic leaf extract of V. lasiopus is beyond 2000 mg/kg body weight. In sub-chronic toxicity studies, V. lasiopus lowered total proteins in all the study groups significantly. Albumin was also lowered at extract dose of 1000 mg/kg body weight. In addition, it resulted to significant neutropenia, lymphocytosis and thrombocytosis in the group administered with dose extract of 1000 mg/kg body weight (P˂0.05). It was therefore concluded that methanolic leaf extract of V. lasiopus is safe for use when administered at therapeutic doses. The plant extract may also be useful in the management of haematological disorders especially thrombocytopenia.en_US
dc.identifier.citationMbinda, W. M., Ngugi, M. P., Koech, S. C., Muthoni, B. G., & Musila, M. N. (2017). Evaluation of In vivo Toxicity of Methanolic Leaf Extract of Vernonia lasiopus (O. Hoffman).en_US
dc.identifier.issn2472-0992
dc.identifier.otherDOI: 10.4172/2472-0992.1000133
dc.identifier.urihttps://www.hilarispublisher.com/open-access/evaluation-of-in-vivo-toxicity-of-methanolic-leaf-extract-of-vernonialasiopus-o-hoffman-2472-0992-1000133.pdf
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/21607
dc.language.isoenen_US
dc.publisherHilaris Publishingen_US
dc.subjectVernonia lasiopusen_US
dc.subjectToxicityen_US
dc.subjectWistar ratsen_US
dc.subjectIn vivoen_US
dc.subjectMethanolic leaf extracten_US
dc.titleEvaluation of In vivo Toxicity of Methanolic Leaf Extract of Vernonia lasiopus (O. Hoffman)en_US
dc.typeArticleen_US
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