Quantitative trait loci (QTL) mapping of resistance to coffee berry disease (Colletotrichum kahawae Waller & Bridge) in Coffea arabica L. variety Rume Sudan

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dc.contributor.authorGimase, James Mwita
dc.contributor.authorThagana, Wilson Muriithi
dc.contributor.authorOmondi, Chrispine Ogutu
dc.contributor.authorCheserek, Jane Jerono
dc.contributor.authorGichimu, Bernard Mukiri
dc.contributor.authorGichuru, Elijah Kathurima
dc.date.accessioned2020-09-17T13:50:03Z
dc.date.available2020-09-17T13:50:03Z
dc.date.issued2020
dc.descriptionA research article published in African Journal of Agricultural Researchen_US
dc.description.abstractCoffee Berry Disease (CBD) is a major constraint that limits Coffea arabica production, whose resistance is governed by three genes, T, R that are dominant and recessive k in varieties Hibrido de Timor (HDT), Rume Sudan (RS) and K7 respectively. This study identified the genomic region occupied by R-gene using F2 genotypes from varieties RS and SL28; and Single Nucleotide Polymorphic (SNP) markers obtained through Genotyping by Sequencing. Redundant markers were removed and 699 markers obtained for linkage mapping and quantitative trait loci (QTL) analysis. The Linkage map spread over 5525.39 cM across eleven coffee chromosomes (Chr). The QTL was analyzed by both Interval Mapping (IM) and Inclusive Composite Interval Mapping (ICIM) using SNP markers and CBD resistance mean scores of the F2 genotypes and their parents. Three QTLs, qCBD 1-1 in Chr 1, qCBD 2-1 and qCBD 2-2 in Chr 2 were significantly associated with CBD resistance, detected by both IM and ICIM at LOD ≥ 2.5 (P≤0.05). Two flanking markers that were closer to the three QTLs; 100025973|F|0-59:T>C- 59:T>C at a distance of 3 centi Morgans (cM) from qCBD 1-1 and 100034991|F|0-44:C>T-44:C>T, that was flanking in both qCBD 2-1 and qCBD 2-2 at 12.5 cM, whose SNPs were significant (P≤0.05), are recommended for validation and use in marker-assisted breeding.en_US
dc.identifier.citationGimase, J. M., Thagana, W. M., Omondi, C. O., Cheserek, J. J., Gichimu, B. M., & Gichuru, E. K. (2020). Quantitative trait loci (QTL) mapping of resistance to coffee berry disease (Colletotrichum kahawae Waller Bridge) in Coffea arabica L. variety Rume Sudan. African Journal of Agricultural Research, 16(8), 1184-1194.en_US
dc.identifier.issn1991-637X
dc.identifier.urihttps://academicjournals.org/journal/AJAR/article-full-text-pdf/5CDC01C64605.pdf
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/20394
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.subjectCoffee berry diseaseen_US
dc.subjectlinkage mapen_US
dc.subjectquantitative trait locien_US
dc.subjectgenotyping by sequencingen_US
dc.subjectsingle nucleotideen_US
dc.subjectpolymorphismen_US
dc.subjectSL 28en_US
dc.subjectR-geneen_US
dc.titleQuantitative trait loci (QTL) mapping of resistance to coffee berry disease (Colletotrichum kahawae Waller & Bridge) in Coffea arabica L. variety Rume Sudanen_US
dc.typeArticleen_US
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