Isolation and biological characterization of non-B HIV type 1 from Kenya

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dc.contributor.authorNgeranwa, J.J.N.
dc.contributor.authorMuriuki, Joseph K
dc.contributor.authorMwangi, Joseph
dc.contributor.authorOrinda, G. O.
dc.contributor.authorLwembe, Raphael
dc.contributor.authorKhamadi, Samuel
dc.date.accessioned2013-05-14T08:55:45Z
dc.date.available2013-05-14T08:55:45Z
dc.date.issued2011-11
dc.description10.1089/AID.2010.0095 pp.660-6en_US
dc.description.abstractThe isolation and characterization of primary strains of human immunodeficiency virus (HIV) is a vital tool for assessing properties of viruses replicating in HIV-infected subjects. HIV-1 isolation was carried out from 30 HIV-1-infected patients from a Comprehensive Care Clinic (CCC) after informed consent. Virus was successfully isolated from 9 out of the 30 samples investigated. Seven of the isolates were from drug-naive patients while two were from patients on antiretroviral drugs. The isolates were biologically phenotyped through measurement of the syncytium-inducing capacity in MT2 cells. Six of the isolates exhibited syncytia induction (SI) associated with CXCR4 coreceptor usage while three of the isolates were non-syncytia-inducing (NSI) isolates associated with CCR5 coreceptor usage. In addition, the replication capacity of the isolates was further determined in established cell line CD4(+) C8166. Indirect immunofluorescence assay was used to check the antigen expression on the cells as a supplementary test. HIV-1 isolation success was 70% (7/10) and 20% (2/20) in naive and drug-experienced patients, respectively. The majority of the viral isolates obtained (6/9) were of the SI phenotype, though SI virus strains are rare among non-B subtypes. A significant correlation between virus isolation success and viral load was established. Coreceptor use data for heavily treatment-experienced patients with limited treatment options are scanty and this is the group with perhaps the most urgent need of novel antiretroviral agents.en_US
dc.identifier.citationAIDS research and human retroviruses (impact factor: 2.18). 11/2011; 28(7):660-6.en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/6791
dc.language.isoenen_US
dc.publisherAIDS research and human retrovirusesen_US
dc.titleIsolation and biological characterization of non-B HIV type 1 from Kenyaen_US
dc.typeArticleen_US
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