Cytolytic activity of CD4+ and CD8+ T lymphocytes in plasmodium falciparum malaria susceptible and resistant individuals in a malaria endemic area of western Kenya

Simple item page
dc.contributor.authorOng'echa, Michael Obor
dc.date.accessioned2012-05-04T12:40:30Z
dc.date.available2012-05-04T12:40:30Z
dc.date.issued2012-05-04
dc.descriptionThe QR 185.3.O5en_US
dc.description.abstractAcquired immunity in malaria is both species and stage-specific, with both B- and T-cells being involved. Cell-mediated immunity by T-cells involves cytokine production and the direct killing of infected hepatocytes and/or sporozoites. This cytolytic ability is effected by cytotoxic T lymphocytes (CTL). One epitope recognized by the CTL (Th3R) spans the amino-acid sequence 368-390 of the circumsporozoite (CS) protein on the surface of the sporozoites and infected hepatocytes. This epitope has significant antigen diversity, thus posing a potential problem in producing a pre-erythocytic vaccine. In this study, peripheral blood lymphcytes (PBL) from adult male residents of a malaria endemic area were expanded for six days using rIL-2 and malaria synthetic peptides representing the 368-390 amino-adi sequence of the Plasmodium falciparum CS protein. The effector cells were then tested against Epstein-Barr virus (EBV) lymphblastoid targets pulsed with or without the peptides. Malaria specific lysis was determined as the difference between lysis in the presence of a peptide and lysis in the absence of a peptide. Specific lysis of 10% or more was considered as a positive response. Samples from 40 individuals were assayed. Ten individuals (25%) showed CTL activity, 13 individuals (32.5 %) showed negative responses and the remaining individuals (42.5%) had 'indeterminate' results. There was no relationship between CTL responses and susceptibility or resistance to P. falciparum re-infection (U=61.5; U0.05(2) 9,13=89; P>0.1). However, there was cross-reactivity of the 368-390 peptide variants. The CTL responses were MHC-restricted, with the short peptide 127 (368-379) being the optimal size for the CTL epitope.en_US
dc.description.sponsorshipKenyatta Universityen_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/4470
dc.language.isoenen_US
dc.subjectMalaria--Kenya, Western--Immunological aspects//Malaria vaccine//Immunityen_US
dc.titleCytolytic activity of CD4+ and CD8+ T lymphocytes in plasmodium falciparum malaria susceptible and resistant individuals in a malaria endemic area of western Kenyaen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Cytolytic activity of.pdf
Size:
70.16 MB
Format:
Adobe Portable Document Format
Description:
Full text thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.68 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
MST-Zoological Sciences