Plant Regeneration from Embryogenic Callus-Derived from Immature Leaves of Momordica Charantia L

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dc.contributor.authorLabod´e Hospice Stevenson, Naïtch´ed´e
dc.contributor.authorAggrey, Bernard Nyende
dc.contributor.authorRuno, Steven
dc.contributor.authorBorlay, Allen Johnny
dc.date.accessioned2024-01-29T09:38:32Z
dc.date.available2024-01-29T09:38:32Z
dc.date.issued2023-11
dc.descriptionArticleen_US
dc.description.abstractBitter melon (Momordica charantia L.), a widely cultivated food and medicinal plant native to the world’s subtropics and tropics, is a Cucurbitaceae rich in carotenoids. However, the low seed germination frequency and progeny variability associated with the production of this plant have a substantial impact on its growth and yield. These constraints affect the availability and exploitation of this crop, especially the fruits, which are rich in secondary metabolites such as β-carotene and α-carotene. In vitro regeneration would help overcome the obstacle linked to the germination of this plant and increase its yield and utilization. A reproducible in vitro organogenesis protocol was established using bitter melon embryogenic callus derived from immature leaf explants of in vivo grown seedlings and in vitro plantlets. Regeneration via callus was conducted on MSB5 media augmented with different plant growth regulator concentrations. The maximum frequency of callus formation (95.09 %) was produced in MSB5 media incorporated with 1.2 mg L− 1 NAA augmented with 0.5 mg L− 1 TDZ. MSB5 medium with no growth regulators was observed to be the most suitable for the shoot and root formation from the callus, producing a significantly high shoot percentage of 90.91 % and 21.53 shoots per explants, and the highest rooting frequency and root number of 88.92 % and 6.23 roots per explant, respectively, from leafderived callus of in vitro plantlets. The elongated plantlets had grown to a significantly higher average height of 12.20 cm on media added with 0.75 mg L− 1 GA3. This reproducible method for regenerating bitter melon plantlets could facilitate mass multiplication, conservation, and commercial field productionen_US
dc.identifier.citationNaïtchédé, L. H. S., Nyende, A. B., Runo, S., & Borlay, A. J. (2023). Plant regeneration from embryogenic callus-derived from immature leaves of Momordica charantia L. Heliyon, 9(11).en_US
dc.identifier.otherhttps://doi.org/10.1016/j.heliyon.2023.e22122
dc.identifier.urihttps://ir-library.ku.ac.ke/handle/123456789/27317
dc.language.isoenen_US
dc.publisherElsevier Ltden_US
dc.subjectCallus inductionen_US
dc.subjectLeaf explantsen_US
dc.subjectBitter melonen_US
dc.subjectRoot inductionen_US
dc.subjectshoot elongationen_US
dc.subjectShoot inductionen_US
dc.titlePlant Regeneration from Embryogenic Callus-Derived from Immature Leaves of Momordica Charantia Len_US
dc.typeArticleen_US
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