In vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesis

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dc.contributor.authorOduor, R.O.
dc.contributor.authorNjagi, E.N.M.
dc.contributor.authorNdung`u, S.
dc.contributor.authorMachuka, Jesse
dc.date.accessioned2012-10-31T12:03:16Z
dc.date.available2012-10-31T12:03:16Z
dc.date.issued2006
dc.description.abstractIn this study, we report successful regeneration of Kenyan white maize using immature embryos derived from three selected Kenyan dryland synthetic maize hybrids; Dryland Hybrid (DH) 02, Pwani Hybrid (PH) 01 and PH04 and an open pollinated variety-OPV (Katumani, KAT). The embryos were cultured in N6 medium supplemented with proline, casein hydrolysate, silver nitrate and 2% sucrose containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and incubated in total darkness at 28±2°C to initiate callus. Mature somatic embryos were sub-cultured in shoot promotion medium consisting of hormone free Murashige and Skoog (MS) media supplemented with 2 mg L-1 glycine and 2% sucrose. Root formation was induced by sub-culturing the shoots on half strength MS medium supplemented with 2% sucrose and 0.1 mg L-1 of Naphthalene Acetic Acid (NAA). Regenerated plantlets were successfully acclimatized in soil and raised to maturity. Fertile progeny were also recovered.en_US
dc.identifier.citationInternational Journal of Botany 2 (2): 146-151, 2006en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/5890
dc.language.isoenen_US
dc.publisherDOAJen_US
dc.subjectIn vitro regenerationen_US
dc.subjectwhite maizeen_US
dc.subjectsomatic embryogenesisen_US
dc.titleIn vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesisen_US
dc.typeArticleen_US
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