Differential Utilization of CCR5 Molecules from Three East African Primate Species by the HIV-1 Envelope Glycoprotein

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dc.contributor.authorMburu, D. N.
dc.date.accessioned2015-07-06T12:04:52Z
dc.date.available2015-07-06T12:04:52Z
dc.date.issued2013
dc.descriptionResearch Articleen_US
dc.description.abstractThis study reports the cloning, sequencing, and functional characterization of novel simian homologs of CCR5 from three species of primates (Papio anubis anubis; Colobus guereza and Cercopithecus neglectus ) which are indigenous to East Africa. Identity at the amino acid level to human CCR5 was 97% for molecules from all three primate species. Functional characterization of these coreceptors was performed using a sensitive gene reporter-based cell-cell fusion assay. Despite a high degree of sequence similarity, significant differences in the degree of M-tropic HIV envelope-specific fusion generated by individual coreceptor molecules were demonstrated. Remarkably, two simian CCR5 molecules (Colobus guereza and Cercopithecus neglectus) demonstrated an increased efficiency of HIV-mediated fusion as compared with the human CCR5 molecule. These results provide further evidence that simian CCR5 molecules differ in their ability to facilitate HIV-1 entry, and may have bearing on the investigation of new simian models for HIV-1 pathogenesis.en_US
dc.description.sponsorshipThis study was facilitated through a collaboration between the the Institute for Primate Research in Nairobi and Vanderbilt AIDS Vaccine Evaluation Unit and the author thanks both instutions for the support that was extended.en_US
dc.identifier.citationInternational Journal of Biotechnology and Biochemistry Volume 9, Number 2 (2013) pp. 197-209en_US
dc.identifier.issn0973-2691
dc.identifier.urihttp://spas.ku.ac.ke/images/stories/docs/research/Differential%20Utilization%20of%20CCR5%20Molecules%20from%20Three%20East%20African%20Primate%20Species%20by%20the%20HIV-1%20Envelope%20Glycoprotein.pdf
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/13107
dc.language.isoenen_US
dc.publisherResearch India Publicationen_US
dc.subjectchemokine receptoren_US
dc.subjectolive baboonen_US
dc.subjectcolobus monkeyen_US
dc.subjectde Brazza monkeyen_US
dc.subjectcell fusion assayen_US
dc.titleDifferential Utilization of CCR5 Molecules from Three East African Primate Species by the HIV-1 Envelope Glycoproteinen_US
dc.typeArticleen_US
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