Protection against Cutaneous Leishmaniasis in Outbred Vervet Monkeys, Using a Recombinant Histone H1 Antigen
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Date
2002-05
Authors
Slavica, Masina
Gicheru, Michael M.
Stéphane, O
Demotz., Nicolas
Fasel, J.
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Infectious Diseases
Abstract
Infection with Leishmania major parasites results in the development of cutaneous ulcerative lesions on the skin. We investigated the protective potential of a single, recombinant histone H1 antigen against cutaneous leishmaniasis in an outbred population of vervet monkeys, using Montanide adjuvant. Protection was assessed by challenging the animals with a mixture of vector sand fly salivary-gland lysate and a low dose of in vitro–derived parasites, thus more closely mimicking natural infection induced by L. major. The course of infection in immunized monkeys was compared with that of animals that had healed from a primary infection and were immune. The monkeys immunized with recombinant histone H1 showed a reduced development of lesion size, compared with controls. Our study therefore illustrates the potential use of histone H1 as a vaccine candidate against cutaneous leishmaniasis in humans
Description
Presented in part: 6th Annual Woods Hole Immunoparasitology Conference, Marine Biological Laboratory, Woods Hole, Massachusetts, 28 April–1 May 2002 (abstract 51)
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Citation
The Journal of Infectious Diseases Volume 188, Issue 8 Pp. 1250-1257