Multidrug-resistant Campylobacter jejuni, Campylobacter coli and Campylobacter lari isolated from asymptomatic school-going children in Kibera slum, Kenya [version 1; peer review: awaiting peer review]
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Date
2020
Authors
Nduhiu, Gitahi
Gathura, Peter B.
Gicheru, Michael M.
Wandia, Beautice M.
Nordin, Annika
Journal Title
Journal ISSN
Volume Title
Publisher
F1000Research
Abstract
Background: The objective of this study was to determine the prevalence
of thermophilic Campylobacter spp. in asymptomatic school-going children
and establish the antibiotic resistant patterns of the isolates towards the
drugs used to treat campylobacteriosis, including macrolides, quinolones
and tetracycline. Campylobacter spp. are a leading cause of enteric illness
and have only recently shown resistant to antibiotics.
Methods: This study isolated Campylobacter spp., including
Campylobacter coli, Campylobacter jejuni and Campylobacter lari, in stool
samples from asymptomatic school-going children in one of the biggest
urban slums in Kenya. The disc diffusion method using EUCAST
breakpoints was used to identify antibiotic-resistant isolates, which were
further tested for genes encoding for tetracycline resistances using
primer-specific polymerase chain reaction.
Results: In total, 580 stool samples were collected from 11 primary schools
considering both gender and age. Subjecting 294 biochemically
characterized Campylobacter spp. isolates to genus-specific PCR, 106
(18.27% of stool samples) isolates were confirmed Campylobacter spp. Out
of the 106 isolates, 28 (4.83%) were Campylobacter coli, 44 (7.58%) were
Campylobacter jejuni while 11 (1.89%) were Campylobacter lari.
Campylobacter jejuni had the highest number of isolates that were
multi-drug resistant, with 26 out of the 28 tested isolates being resistant to
ciprofloxacin (5 mg), nalidixic acid (30 mg), tetracycline (30 mg) and
erythromycin (15 mg).
Conclusions: In conclusion, a one-health approach, which considers
overlaps in environment, animals and human ecosystems, is recommended
in addressing campylobacteriosis in humans, since animals are the main
reservoirs and environmental contamination is evident.
Description
Research paper
Keywords
Multidrug, resistance, Campylobacter, genes, asymptomatic
Citation
F1000Research, 2020