Analgesic Potential of Acetone Leaf Extract of Caesalpinia volkensii Harms in Mice
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Date
2015
Authors
Mworia, J.K.
Gitahi, S.M.
Juma, K.K.
Njagi, J.M.
Mwangi, B.M.
Aliyu, U.
Njoroge, W.A.
Mwonjoria, K.J.
Nyamai, D.W.
Ngugi, M.P.
Journal Title
Journal ISSN
Volume Title
Publisher
OMICS International
Abstract
Pain is defined as unpleasant feeling essential for body’s defense system. It acts as a warning signal against
disturbances in the body. Conventional antinociceptives are expensive and have many side effects. Continued use
of these drugs may lead to tolerance and resistance. Medicinal plants have been used to relieve pain and form a
better alternative. Herbal antinociceptives are affordable and have arguably fewer side effects. Caesalpinia volkensii
(Harms) has pharmacological activities that include antimicrobial, immune modulatory properties and antimalarial.
It is used locally by people in Embu County as analgesics. This study was designed to bioscreen the acetone leaf
extracts of C. volkensii (Harms) for antinociceptive effects in mice.. The plant parts were collected from Mbeere
north sub-county, Embu County, Kenya. The samples were prepared and extraction of the active compounds carried
out using acetone. Swiss albino mice were divided into five groups of five mice each: Normal, negative, reference
and experimental group. Pain was induced experimentally using formalin and acetic acid. The experimental groups
were treated with 50 and 100 mg/kg dose ranges of the plant extract. Mice were injected intraperitoneally with
doses of the herbs, diclofenac and the vehicle. Thirty minutes later the animals were injected with 0.01ml of 2.5 %
formalin in the sub planter region of the left hind paw and the other set with 0.4ml of 5% acetic acid. The total time
spent lifting; biting, licking the paw and writhing were counted and scored. The acetone leaves extracts tested at
different dose levels lowered paw licking time in a dose dependant manner, Further, the phytochemical screening
results showed that the acetone leaves extracts of C. volkensii (Harms) have phytochemicals associated with antinociceptive
activities. The study has established that the acetone leaves extract of C.volkensii (Harms) is effective
in management of pain.
Description
DOI:10.4172/21532435.1000450
Keywords
Caesalpinia volkensii (Harms), writhing, Antinociceptive, Formalin, Acetic acid
Citation
Pharm Anal Acta Volume 6 Issue 11; 2015