Transformation of Tropical Maize with the NPK1 Gene for Drought Tolerance

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dc.contributor.authorMatheka, J. M.
dc.contributor.authorOmer, Rasha A
dc.contributor.authorMachuka, Jesse
dc.contributor.authorAli, Abdelbagi M.
dc.date.accessioned2015-04-22T09:38:21Z
dc.date.available2015-04-22T09:38:21Z
dc.date.issued2013
dc.descriptionDOI: 10.5923/j.ijge.20130302.01en_US
dc.description.abstractThe objective of this study was to determine amenability of different tropical maize genotypes to Agrobacterium-mediated transformation with the NPK1 gene for conference of drought tolerance. To achieve this, immature embryos from thirteen maize genotypes (IL1, IL3, IL15, IL16, IL28, IL38, IL42, IL43, Hudiba-1, Hudiba-2, Mojtamma-45, A188 and KAT) were transformed by cocultivating with Agrobacterium tumefaciens strain EHA101 harboring the pSHX004 vector. Transgenic tussues were recovered on PPT (3 mg/l)-containing medium. Statistically significant differences (p<0.05) were observed between the genotypes with respect to transformation frequency (TF). Overall, IL3 was identified as the most amenable to transformation with a TF of 31.7% and proved to be superior to A188, which recorded a TF of 5.82%. Hudiba-2 was identified as the most transformable open pollinated variety (OPV) with a TF of 8.7% compared to that of 7.3% for KAT. IL1 and Mojtamma-45 proved to be poor responders to transformation with TFs of 2.5% and 1.7%, respectively. Putative transgenics were recovered from IL3, IL15, Hudiba-2, IL1, IL38, Hudiba-1, A188 and KAT. The frequency of regeneration of PPT resistant shoots varied from 6% for A188 to 100% for Hudiba-2. Stable integration of the transgene was confirmed by PCR. In conclusion we have demonstrated that tropical maize genotypes adapted to Sudan are transformable with the NPK1en_US
dc.description.sponsorshipThe work done at the Plant transformation Laboratory at Kenyatta University under ASARECA funding. We would like to thank Professor Kang Wang from IOWA State University for providing the gene construct. We acknowledge the Agricultural research corporation of Sudan for providing the maize germplasm used in this study.en_US
dc.identifier.citationInternational Journal of Genetic Engineering 2013, 3(2): 7-14 DOI: 10.5923/j.ijge.20130302.01en_US
dc.identifier.issn2167-7239
dc.identifier.issn2167-7220
dc.identifier.urihttp://article.sapub.org/10.5923.j.ijge.20130302.01.html
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/12517
dc.language.isoenen_US
dc.publisherScientific & Academic Publishingen_US
dc.subjectTropical Maizeen_US
dc.subjectAgrobacterium-Mediated Transformationen_US
dc.subjectNPK1en_US
dc.subjectDrought Toleranceen_US
dc.titleTransformation of Tropical Maize with the NPK1 Gene for Drought Toleranceen_US
dc.typeArticleen_US
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