Safety and Skin Delayed-type Hypersensitivity Response in Vervet Monkeys Immunized with Leishmania Donovani Sonicate Antigen Delivered with Adjuvants

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dc.contributor.authorMutiso, J. M.
dc.contributor.authorMacharia, J. C.
dc.contributor.authorTaracha, E.
dc.contributor.authorWafula, K.
dc.contributor.authorRikoi, H.
dc.contributor.authorGicheru, M. M.
dc.date.accessioned2013-12-04T15:33:55Z
dc.date.available2013-12-04T15:33:55Z
dc.date.issued2013-01
dc.description.abstractIn this study, we report on the safety and skin delayed-type hypersensitivity (DTH), responses of the Leishmania donovani whole cell sonicate antigen delivered in conjunction with alum-BCG (AlBCG), Montanide ISA 720 (MISA) or Monophosphoryl lipid A (MPLA) in groups of vervet monkeys. Following three intradermal injections of the inoculums on days 0, 28 and 42, safety and DTH responses were assessed. Preliminary tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) levels were also measured and these were compared with DTH. Only those animals immunized with alum-BCG reacted adversely to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric analysis of variance followed by a post-test showed significantly higher DTH responses in the MISA+Ag group compared with other immunized groups (p < 0.001). The MPLA+Ag group indicated significantly lower DTH responses to the sonicate antigen compared with the AlBCG+Ag group. There was a significant correlation between the DTH and cytokine responses (p < 0.0001). Based on this study we conclude that Leishmania donovani sonicate antigen containing MISA 720 is safe and is associated with a strong DTH reaction following immunization.en_US
dc.identifier.citationRevista do Instituto de Medicina Tropical de Sao Paulo;Jan/Feb2012, Vol. 54 Issue 1, p37en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/7801
dc.language.isoenen_US
dc.publisherInstitute of Primate Researchen_US
dc.subjectSafetyen_US
dc.subjectDelayed-type hypersensitivity (DTH)en_US
dc.subjectVervet monkeysen_US
dc.subjectLeishmania donovani sonicate antigenen_US
dc.subjectAdjuvantsen_US
dc.titleSafety and Skin Delayed-type Hypersensitivity Response in Vervet Monkeys Immunized with Leishmania Donovani Sonicate Antigen Delivered with Adjuvantsen_US
dc.typeArticleen_US
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