Safety and Skin Delayed-type Hypersensitivity Response in Vervet Monkeys Immunized with Leishmania Donovani Sonicate Antigen Delivered with Adjuvants
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Date
2013-01
Authors
Mutiso, J. M.
Macharia, J. C.
Taracha, E.
Wafula, K.
Rikoi, H.
Gicheru, M. M.
Journal Title
Journal ISSN
Volume Title
Publisher
Institute of Primate Research
Abstract
In this study, we report on the safety and skin delayed-type hypersensitivity (DTH), responses of the Leishmania donovani whole cell sonicate antigen delivered in conjunction with alum-BCG (AlBCG), Montanide ISA 720 (MISA) or Monophosphoryl lipid A (MPLA) in groups of vervet monkeys. Following three intradermal injections of the inoculums on days 0, 28 and 42, safety and DTH responses were assessed. Preliminary tumor necrosis factor alpha (TNF-α) and interferon gamma (IFN-γ) levels were also measured and these were compared with DTH. Only those animals immunized with alum-BCG reacted adversely to the inoculum by producing ulcerative erythematous skin indurations. Non-parametric analysis of variance followed by a post-test showed significantly higher DTH responses in the MISA+Ag group compared with other immunized groups (p < 0.001). The MPLA+Ag group indicated significantly lower DTH responses to the sonicate antigen compared with the AlBCG+Ag group. There was a significant correlation between the DTH and cytokine responses (p < 0.0001). Based on this study we conclude that Leishmania donovani sonicate antigen containing MISA 720 is safe and is associated with a strong DTH reaction following immunization.
Description
Keywords
Safety, Delayed-type hypersensitivity (DTH), Vervet monkeys, Leishmania donovani sonicate antigen, Adjuvants
Citation
Revista do Instituto de Medicina Tropical de Sao Paulo;Jan/Feb2012, Vol. 54 Issue 1, p37