Antimicrobial Resistance Genes in Salmonella and Escherichia Coli Isolates from Chicken Droppings in Nairobi, Kenya
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Date
2019
Authors
Langata, Lydia Mali
Maingi, John M.
Musonye, Harry Asena
Kiiru, John
Nyamache, Anthony Kebira
Journal Title
Journal ISSN
Volume Title
Publisher
Cross Mark
Abstract
Objective: Increase in antimicrobial resistance is a threat to health sector globally. Surveillance on the spread and
emergence of antimicrobial resistance is therefore invertible. This study investigated prevalence of Salmonella and
Escherichia coli, molecularly characterized their antimicrobial resistance patterns and spread among resistant isolates
from chicken droppings.
Results: A total of 150 chicken households were selected randomly within Nairobi and fresh chicken droppings collected.
Salmonella and Escherichia coli were isolated and antimicrobial susceptibility test carried out. Beta-lactamase
genes and class 1 integrons were determined among amoxicillin resistant isolates. Isolates carrying TEM gene were
further subjected to (GTG)5 PCR genotyping. Of the analysed samples, 57% and 12% contained Escherichia coli and
Salmonella respectively. Most of the isolates were susceptible to the tested antibiotics with exemption of 53% of the
isolates that were resistant to amoxicillin. The isolates were detected with TEM (46%), CTX-M (18%) resistance genes
and class 1 integrons (25%). The study reveals presence of beta-lactamase genes and class 1 integrons across Salmonella
and Escherichia coli isolates from droppings of reared chicken. Therefore, the wide distribution of chicken and
their fecal waste is likely to increase development of antibiotic resistance.
Description
Research Article
Keywords
Beta-lactamase genes, Class 1 integrons, Clonal expansion
Citation
Langata, L. M., Maingi, J. M., Musonye, H. A., Kiiru, J., & Nyamache, A. K. (2019). Antimicrobial resistance genes in Salmonella and Escherichia coli isolates from chicken droppings in Nairobi, Kenya. BMC research notes, 12(1), 1-6.