Structure, expression profile, and evolution of the sucrose synthase gene family in peach (Prunus persica)
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Date
2015-03
Authors
Korir, Nicholas Kibet
Chunhua Zhang
Mingliang Yu
Ruijuan Ma
Zhijun Shen
Binbin Zhang
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Verlag
Abstract
Key message SUS gene family is comprised of six genes
in peach, PpSus1 to PpSus6. PpSus1 to PpSus6 were
categorized to represent three groups, group I, II, and
III. PpSus showed conservative characteristics with Sus
in other plants. PpSus exhibited distinct expression patterns
in tissues at four development stages.
Abstract Sucrose synthase (SUS) has been suggested to
play a key role in plant sucrose metabolism with recent
studies reporting that a small number of genes encoding
different isozymes of Sus exist in most plant species. Despite
this, information on genes encoding different isozymes
of Sus in peach (Prunus persica) is scanty. In this
study, we report the prediction, isolation, structural characteristics,
phylogenetic connections and expression outline
of six Sus genes in peach (PpSus1 to 6). The six PpSus
genes were found distributed across scaffolds 1, 3, 5, 7, and
8. Analysis of the exons/introns revealed that PpSus genes
contain multiple introns that range from 11 to 13 and displayed
a high degree of conservation with corresponding
Sus genes in other plant species. The comparative
screening of motifs in PpSus proteins indicated high conservation
in terms of number, width and order of motifs
among PpSus proteins, which indirectly indicates that the
six PpSus proteins are indeed members of the SUS family.
Phylogenetic analysis revealed that PpSus2 to PpSus4 belonged
to group II of the Sus family, PpSus5 and PpSus6
were clustered into group III, and group I contained only
one peach gene (PpSus1) together with members from 10
other plant species. Analysis of expression levels of the six
PpSus genes revealed that transcripts of PpSus1 were almost
undetectable in leaves and in older phloem, while
PpSus2 and PpSus4 were almost undetectable in flowers.
The other three PpSus genes appeared differentially expressed
in all tissues examined and were detected at different
stages of tissue development. The results obtained
from this study will be useful in selecting candidate PpSus
genes for further functional analysis in the pathway of
sucrose metabolism in peach and specifically in characterizing
the knockout/knockdown mutants of PpSus genes
Description
Article
Keywords
Peach, Sucrose synthase, Characteristics, Phylogenetic analysis, Expression pattern
Citation
Acta Physiol Plant (2015) 37:81
URI
http://download-v2.springer.com/static/pdf/992/art%253A10.1007%252Fs11738-015-1829-4.pdf?token2=exp=1432041828~acl=%2Fstatic%2Fpdf%2F992%2Fart%25253A10.1007%25252Fs11738-015-1829-4.pdf*~hmac=e50861450413c3e535708b333b09d623fe95da00d3a1105ad47e15590139faec
http://ir-library.ku.ac.ke/handle/123456789/12642
http://ir-library.ku.ac.ke/handle/123456789/12642