Anti-inflammatory activities of dichloromethane-methanolic leaf and stem bark extracts of Ximenia americana in mice models

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dc.contributor.authorGaichu, Daniel M.
dc.contributor.authorNthiga, Peter M.
dc.contributor.authorKariuki, Duncan M.
dc.contributor.authorNgugi, M.P.
dc.contributor.authorMburu, David N.
dc.date.accessioned2017-12-30T11:14:23Z
dc.date.available2017-12-30T11:14:23Z
dc.date.issued2017
dc.descriptionResearch Articleen_US
dc.description.abstractIntroduction: Ximenia americana is a highly branched shrub mainly found in tropics of Asia, Africa, New Zealand, Central and South America among others. In most parts of Africa, X. americana is used in folklore to treat various disorders such as oedema, pain, fever, helminthiasis, diarrhea and burns among others. There is no published data on anti-inflammatory activities of organic extracts of X. americana. It is against this background that this research was carried out. The study tested for the anti-inflammatory activities of dichloromethane-methanolic (DCM-MeOH) leaf and stem bark extracts of X. americana in rats. Methods: The plant materials were collected from Mbeere North sub-county, Embu county, Kenya. Methanol and dichloromethane in the ratio of 1:1 was used to extract the active compounds. Five to 6 weeks old Swiss Albino mice were employed for the anti-inflammatory studies. Animals were divided into 6 groups of 5 mice each: normal, negative, reference and three experimental groups (50, 100 and 150 mg/kg body weight). Inflammation was induced experimentally using carrageenan. The experimental groups were treated with predetermined dose quantities of prepared extracts. Diclofenac was used as the reference drug. Data was analyzed using one-way analysis of variance (ANOVA). Results: The extracts from the leaves reduced hind paw circumference by between 0.91% and 16.90% while the stem bark extracts reduced hind paw circumference by between 5.84% and 29.00%. Diclofenac reduced right hind paw circumference by 1.32%-29.60%. Qualitative phytochemical screening showed presence of alkaloids, flavonoids, steroids, saponins, cardiac glycosides, phenolics and terpenoids in the extract. Conclusion: The study established that the DCM-MeOH leaf and stem bark extracts of X. americana is effective in management of inflammation and therefore it can be explored as a possible bio-resource in the development of herbal medicines.en_US
dc.identifier.citationGaichu DM, Nthiga PM, Kariuki DM, Ngugi MP, Mburu DN. Anti-inflammatory activities of dichloromethane-methanolic leaf and stem bark extracts of Ximenia americana in mice models. J Herbmed Pharmacol. 2017;6(2):55-61.en_US
dc.identifier.urihttp://ir-library.ku.ac.ke/handle/123456789/18062
dc.language.isoenen_US
dc.publisherShahrekord University of Medical Sciencesen_US
dc.subjectXimenia americanaen_US
dc.subjectDCM-MeOHen_US
dc.subjectAnti-inflammationen_US
dc.subjectHind pawen_US
dc.subjectDiclofenacen_US
dc.subjectCarrageenanen_US
dc.titleAnti-inflammatory activities of dichloromethane-methanolic leaf and stem bark extracts of Ximenia americana in mice modelsen_US
dc.typeArticleen_US
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